Detailed information of TA system TA047691

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1724056..1724844
• Replicon: chromosome
• Accession: NZ_CP008841
• Organism: Klebsiella michiganensis strain M1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HR38_RS08090
• Protein ID: WP_038424005.1
• Coordinates: 1724467..1724844 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HR38_RS08085
• Protein ID: WP_038424004.1
• Coordinates: 1724056..1724415 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR38_RS08040	1719119..1719847	+	729	WP_038423996.1	DUF3944 domain-containing protein	-
HR38_RS08045	1719907..1720584	+	678	WP_038423997.1	WYL domain-containing protein	-
HR38_RS08050	1720638..1721102	+	465	WP_038423998.1	hypothetical protein	-
HR38_RS08055	1721157..1721516	+	360	WP_038423999.1	hypothetical protein	-
HR38_RS08060	1721597..1721809	+	213	WP_010434180.1	hypothetical protein	-
HR38_RS08065	1721920..1722741	+	822	WP_038424000.1	DUF945 domain-containing protein	-
HR38_RS08070	1722821..1723306	+	486	WP_038424001.1	antirestriction protein	-
HR38_RS08075	1723318..1723797	+	480	WP_038424002.1	DNA repair protein RadC	-
HR38_RS08080	1723812..1724033	+	222	WP_038424003.1	DUF987 domain-containing protein	-
HR38_RS08085	1724056..1724415	+	360	WP_038424004.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HR38_RS08090	1724467..1724844	+	378	WP_038424005.1	TA system toxin CbtA family protein	Toxin
HR38_RS08095	1724841..1725332	+	492	WP_038424006.1	hypothetical protein	-
HR38_RS08100	1725362..1725565	+	204	WP_038424007.1	DUF957 domain-containing protein	-
HR38_RS08105	1725625..1726602	-	978	WP_051800673.1	glycosyltransferase	-
HR38_RS08110	1727074..1727907	+	834	WP_038424008.1	DUF4942 domain-containing protein	-
HR38_RS08115	1727978..1728985	+	1008	WP_038424009.1	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14204.11 Da
• Isoelectric Point: 5.7237

>T47691 WP_038424005.1 NZ_CP008841:1724467-1724844 [Klebsiella michiganensis]
MQTQQLSSTEETSPRPSPVEIWQQLLSYLLDRHYGLTLNDTPFGNDGVIQEHIEAGISLCDAVNFIVEKYALVRIERRGF
NAETQSPLLSSIDILRARKATGLMTRNGYRMVTDITTGKYREMQP

Nucleotide

• Length: 378 bp

>T47691 NZ_CP008841:1724467-1724844 [Klebsiella michiganensis]
ATGCAAACTCAACAACTATCCTCAACGGAGGAGACCTCACCACGTCCGTCACCGGTGGAGATCTGGCAACAGCTTCTGAG
CTACCTGCTGGATCGTCACTACGGCCTGACGCTCAACGACACGCCTTTCGGCAATGATGGTGTGATCCAGGAACATATCG
AAGCCGGTATCTCACTGTGCGACGCCGTGAATTTTATCGTGGAAAAGTACGCCCTGGTGCGCATCGAACGTCGCGGTTTT
AACGCAGAAACTCAGTCGCCGCTTCTCTCCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACGCGCAA
CGGCTACAGAATGGTAACCGATATCACCACCGGCAAATACCGCGAGATGCAGCCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13224.98 Da
• Isoelectric Point: 5.0063

>AT47691 WP_038424004.1 NZ_CP008841:1724056-1724415 [Klebsiella michiganensis]
MQNTTNVANHDIAEPWWGLKPTVTPCFGTRLVQEGNRLHYLADRASITGTFSDADLRHLDQTFPQLLKQLELMLVSGELN
PRYQDCVTLYTKGLTCEADSLGSCGYVYIALYPTPATTA

Nucleotide

• Length: 360 bp

>AT47691 NZ_CP008841:1724056-1724415 [Klebsiella michiganensis]
ATGCAGAACACAACAAACGTAGCAAATCATGATATTGCCGAACCCTGGTGGGGACTTAAACCCACCGTCACGCCGTGTTT
TGGCACACGCCTGGTGCAGGAAGGCAACCGCCTGCATTACCTGGCTGACCGCGCCAGTATCACCGGCACCTTTAGTGACG
CGGATTTACGCCATCTCGACCAGACATTTCCGCAACTGCTCAAACAGCTTGAACTGATGCTGGTTTCCGGTGAACTCAAT
CCCCGCTATCAGGACTGTGTCACTCTCTACACAAAAGGGCTGACCTGCGAGGCCGACAGCCTGGGTAGTTGCGGCTATGT
CTATATTGCGCTTTACCCAACACCTGCAACAACCGCGTAA