Detailed information of TA system TA047687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2550240..2550777
• Replicon: chromosome
• Accession: NZ_CP008835
• Organism: Burkholderia pseudomallei strain BGR

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: DP60_RS28835
• Protein ID: WP_009923450.1
• Coordinates: 2550240..2550509 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DP60_RS28840
• Protein ID: WP_004525420.1
• Coordinates: 2550493..2550777 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP60_RS28810	2546155..2547858	+	1704	WP_004553173.1	site-specific integrase	-
DP60_RS28815	2548035..2548301	-	267	Protein_2055	transposase	-
DP60_RS28825	2548371..2549491	+	1121	WP_038802333.1	IS3 family transposase	-
DP60_RS28830	2549647..2550105	-	459	Protein_2057	prohibitin family protein	-
DP60_RS28835	2550240..2550509	+	270	WP_009923450.1	BrnT family toxin	Toxin
DP60_RS28840	2550493..2550777	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DP60_RS28845	2551424..2551807	+	384	Protein_2060	DDE-type integrase/transposase/recombinase	-
DP60_RS38270	2551770..2551970	+	201	Protein_2061	IS110 family transposase	-
DP60_RS38275	2553205..2553438	+	234	Protein_2063	transposase	-
DP60_RS28860	2553665..2553781	-	117	Protein_2064	IS3 family transposase	-
DP60_RS28865	2554047..2555507	+	1461	WP_004538070.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2521187..2555507	34320
	-	inside	IScluster/Tn	-	-	2548035..2559150	11115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T47687 WP_009923450.1 NZ_CP008835:2550240-2550509 [Burkholderia pseudomallei]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T47687 NZ_CP008835:2550240-2550509 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47687 WP_004525420.1 NZ_CP008835:2550493-2550777 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47687 NZ_CP008835:2550493-2550777 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8