Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-RelB |
| Location | 220188..220714 | Replicon | plasmid pKPC-272 |
| Accession | NZ_CP008825 | ||
| Organism | UNVERIFIED_ORG: Enterobacter cloacae ECNIH2 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | S1EXL1 |
| Locus tag | ECNIH2_RS26115 | Protein ID | WP_000323025.1 |
| Coordinates | 220188..220475 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | S1F6D3 |
| Locus tag | ECNIH2_RS26120 | Protein ID | WP_000534858.1 |
| Coordinates | 220475..220714 (-) | Length | 80 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECNIH2_RS29070 | 215758..215934 | - | 177 | WP_001371930.1 | hypothetical protein | - |
| ECNIH2_RS26090 (ECNIH2_26280) | 216445..217389 | + | 945 | WP_000778029.1 | DUF5417 domain-containing protein | - |
| ECNIH2_RS26095 (ECNIH2_26285) | 217485..218087 | + | 603 | WP_012695474.1 | hypothetical protein | - |
| ECNIH2_RS26100 (ECNIH2_26290) | 218147..218497 | + | 351 | WP_000743059.1 | hypothetical protein | - |
| ECNIH2_RS26105 | 218544..218747 | + | 204 | WP_001015183.1 | hypothetical protein | - |
| ECNIH2_RS26110 (ECNIH2_26300) | 219029..219349 | + | 321 | WP_000332796.1 | hypothetical protein | - |
| ECNIH2_RS27990 | 219962..220087 | - | 126 | WP_229020258.1 | DUF5431 family protein | - |
| ECNIH2_RS26115 (ECNIH2_26310) | 220188..220475 | - | 288 | WP_000323025.1 | type II toxin-antitoxin system mRNA interferase RelE | Toxin |
| ECNIH2_RS26120 (ECNIH2_26315) | 220475..220714 | - | 240 | WP_000534858.1 | type II toxin-antitoxin system antitoxin RelB | Antitoxin |
| ECNIH2_RS29330 | 220739..220843 | + | 105 | Protein_230 | hypothetical protein | - |
| ECNIH2_RS26125 (ECNIH2_26320) | 220977..221900 | - | 924 | WP_000167917.1 | cation diffusion facilitator family transporter | - |
| ECNIH2_RS26130 (ECNIH2_26325) | 222100..222672 | - | 573 | WP_001515348.1 | cytochrome b/b6 domain-containing protein | - |
| ECNIH2_RS26140 (ECNIH2_26330) | 223148..224386 | - | 1239 | WP_000219087.1 | IS110-like element ISEsa2 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1B / blaKPC-3 / ant(2'')-Ia / catB3 / qacE / sul1 / mcr-9 / aph(3')-Ia | - | 1..282439 | 282439 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11225.22 Da Isoelectric Point: 10.1967
>T47644 WP_000323025.1 NZ_CP008825:c220475-220188 [Enterobacter cloacae ECNIH2]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
Download Length: 288 bp
>T47644 NZ_CP008825:c220475-220188 [Enterobacter cloacae ECNIH2]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA
Antitoxin
Download Length: 80 a.a. Molecular weight: 9071.48 Da Isoelectric Point: 4.5230
>AT47644 WP_000534858.1 NZ_CP008825:c220714-220475 [Enterobacter cloacae ECNIH2]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
Download Length: 240 bp
>AT47644 NZ_CP008825:c220714-220475 [Enterobacter cloacae ECNIH2]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|