Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-YefM
Location 298877..299448 Replicon plasmid pKEC-39c
Accession NZ_CP008824
Organism UNVERIFIED_ORG: Enterobacter cloacae ECNIH2

Toxin (Protein)


Gene name parE Uniprot ID A0A2I8S8S5
Locus tag ECNIH2_RS27860 Protein ID WP_011918375.1
Coordinates 299137..299448 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1Z3MW28
Locus tag ECNIH2_RS24915 Protein ID WP_011787805.1
Coordinates 298877..299137 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH2_RS24890 (ECNIH2_25060) 294086..294670 + 585 Protein_326 3'-5' exonuclease -
ECNIH2_RS24895 (ECNIH2_25065) 295167..296657 + 1491 WP_011787801.1 SulP family inorganic anion transporter -
ECNIH2_RS24900 (ECNIH2_25070) 296692..297546 + 855 WP_011787802.1 universal stress protein -
ECNIH2_RS24905 (ECNIH2_25075) 297637..297969 + 333 WP_011787803.1 TraR/DksA C4-type zinc finger protein -
ECNIH2_RS24910 (ECNIH2_25080) 298087..298707 - 621 WP_011787804.1 recombinase family protein -
ECNIH2_RS24915 (ECNIH2_25085) 298877..299137 + 261 WP_011787805.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ECNIH2_RS27860 299137..299448 + 312 WP_011918375.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECNIH2_RS24920 (ECNIH2_25090) 299472..302552 + 3081 WP_011787830.1 Tn3 family transposase -
ECNIH2_RS24925 (ECNIH2_25095) 302606..302896 + 291 Protein_334 3'-5' exonuclease -
ECNIH2_RS24930 (ECNIH2_25100) 302893..303384 + 492 WP_000139717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaFOX-5 / dfrA14 / ant(2'')-Ia / qacE / sul1 / msr(E) / mph(E) / blaKPC-2 / blaSHV-5 / aac(3)-Ia / qnrA1 - 1..319976 319976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-92)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-61)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11862.70 Da        Isoelectric Point: 8.5341

>T47642 WP_011918375.1 NZ_CP008824:299137-299448 [Enterobacter cloacae ECNIH2]
MPVTYHLTPDAQSDLIGIHRFTLAQWGTTQSKTYLSGLRQTIQLLAETPTLGKNRPEVRMNVFSFPYSSHVIYYIQHEHQ
FVVFGILHKSMVPLAHLAEREII

Download         Length: 312 bp

>T47642 NZ_CP008824:299137-299448 [Enterobacter cloacae ECNIH2]
ATGCCGGTAACTTATCATTTAACGCCCGATGCACAATCTGATCTGATAGGTATTCACCGCTTTACGTTAGCCCAATGGGG
AACGACTCAGTCGAAGACCTATTTATCAGGACTTAGACAAACGATTCAACTGCTGGCTGAAACACCCACCCTTGGGAAAA
ATAGACCCGAGGTACGCATGAATGTGTTTAGCTTCCCTTATTCAAGTCATGTCATCTATTACATCCAACATGAGCATCAA
TTCGTTGTATTTGGGATCTTACATAAAAGTATGGTTCCACTTGCACATCTCGCGGAGCGGGAAATTATTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9312.71 Da        Isoelectric Point: 6.4546

>AT47642 WP_011787805.1 NZ_CP008824:298877-299137 [Enterobacter cloacae ECNIH2]
MNTLSANEAKIHFGDLLLKAQQAPIQINKNGKPVAVVISADAYQSIETLKLHLLQSKAVKAITDIKMGNLVDGNTFFDEL
AAGQYD

Download         Length: 261 bp

>AT47642 NZ_CP008824:298877-299137 [Enterobacter cloacae ECNIH2]
ATGAACACTCTTTCAGCCAATGAAGCAAAAATTCACTTTGGAGATTTGTTGCTCAAAGCTCAACAAGCTCCAATCCAAAT
TAATAAAAATGGCAAGCCTGTGGCCGTTGTTATTTCAGCTGATGCATACCAAAGTATCGAGACACTAAAATTACATTTGC
TTCAATCCAAAGCGGTGAAAGCCATAACTGACATCAAAATGGGCAATTTGGTTGATGGTAATACCTTTTTTGATGAGCTG
GCCGCAGGGCAGTATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S8S5

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3MW28

References