Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2896513..2897118 Replicon chromosome
Accession NZ_CP008823
Organism UNVERIFIED_ORG: Enterobacter cloacae ECNIH2

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag ECNIH2_RS13770 Protein ID WP_012906750.1
Coordinates 2896513..2896692 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A822X3T2
Locus tag ECNIH2_RS13775 Protein ID WP_032619507.1
Coordinates 2896714..2897118 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH2_RS13750 (ECNIH2_13790) 2892442..2892765 - 324 WP_017384434.1 hypothetical protein -
ECNIH2_RS29180 2892768..2893160 - 393 Protein_2715 DUF4225 domain-containing protein -
ECNIH2_RS29185 2893182..2893523 - 342 WP_223862348.1 DUF4225 domain-containing protein -
ECNIH2_RS13760 (ECNIH2_13800) 2894425..2894958 - 534 WP_032619510.1 glycosyltransferase family A protein -
ECNIH2_RS13765 (ECNIH2_13805) 2894955..2896229 - 1275 WP_032619509.1 ATP-binding protein -
ECNIH2_RS13770 (ECNIH2_13810) 2896513..2896692 + 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
ECNIH2_RS13775 (ECNIH2_13815) 2896714..2897118 + 405 WP_032619507.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECNIH2_RS13780 (ECNIH2_13820) 2897159..2898229 - 1071 WP_032619506.1 glycosyltransferase family 9 protein -
ECNIH2_RS13785 (ECNIH2_13825) 2898306..2898884 - 579 WP_032619505.1 tail fiber assembly protein -
ECNIH2_RS26745 (ECNIH2_13830) 2898884..2901061 - 2178 WP_050019395.1 phage tail protein -
ECNIH2_RS13795 (ECNIH2_13835) 2901064..2901615 - 552 WP_032619504.1 phage tail protein I -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fliA / gtrA / gtrB 2890140..3003383 113243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T47636 WP_012906750.1 NZ_CP008823:2896513-2896692 [Enterobacter cloacae ECNIH2]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T47636 NZ_CP008823:2896513-2896692 [Enterobacter cloacae ECNIH2]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTTAGAGTGAAAGGCAGTCATCACCAGTT
TAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATTA
AAAAACAGGCGGGGCTCTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14676.53 Da        Isoelectric Point: 4.2136

>AT47636 WP_032619507.1 NZ_CP008823:2896714-2897118 [Enterobacter cloacae ECNIH2]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGEDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVINQR

Download         Length: 405 bp

>AT47636 NZ_CP008823:2896714-2897118 [Enterobacter cloacae ECNIH2]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGAAGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCCGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAGTACGCCGGTGCGGTATGGGCCCTAGTTGAC
GTGGATATAACACGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTACCCTCATTACTGATCCGACGTATTGATCA
GTTTGTGGCCGCGCATCCTGAATATGGAAGCCGTTCGGGCTTCCTTTCTCGCGTCGCTGCTGACAAGGTTATTAATCAAC
GATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A822X3T2

References