Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2420877..2421616 Replicon chromosome
Accession NZ_CP008823
Organism UNVERIFIED_ORG: Enterobacter cloacae ECNIH2

Toxin (Protein)


Gene name tacT Uniprot ID A0A822W909
Locus tag ECNIH2_RS11550 Protein ID WP_017382345.1
Coordinates 2420877..2421362 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag ECNIH2_RS11555 Protein ID WP_003857131.1
Coordinates 2421350..2421616 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH2_RS27100 2416091..2416306 - 216 Protein_2265 phosphotransferase -
ECNIH2_RS11525 (ECNIH2_11560) 2416364..2417332 - 969 WP_013815099.1 IS5-like element IS903B family transposase -
ECNIH2_RS11530 (ECNIH2_11565) 2417364..2417735 - 372 Protein_2267 IS5/IS1182 family transposase -
ECNIH2_RS11535 (ECNIH2_11570) 2417810..2418442 + 633 WP_001567369.1 hypothetical protein -
ECNIH2_RS11540 (ECNIH2_11575) 2418471..2419874 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
ECNIH2_RS11545 (ECNIH2_11580) 2420257..2420826 + 570 WP_017382346.1 hypothetical protein -
ECNIH2_RS11550 (ECNIH2_11585) 2420877..2421362 - 486 WP_017382345.1 GNAT family N-acetyltransferase Toxin
ECNIH2_RS11555 (ECNIH2_11590) 2421350..2421616 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
ECNIH2_RS11560 (ECNIH2_11595) 2421680..2422609 - 930 WP_023303744.1 LysR family transcriptional regulator -
ECNIH2_RS11565 (ECNIH2_11600) 2422739..2424127 + 1389 WP_032619651.1 MFS transporter -
ECNIH2_RS11570 (ECNIH2_11605) 2424149..2425144 - 996 WP_023303746.1 DUF2891 domain-containing protein -
ECNIH2_RS11575 (ECNIH2_11610) 2425154..2426140 - 987 WP_017382341.1 DUF979 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2405652..2421616 15964


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17510.21 Da        Isoelectric Point: 9.9658

>T47627 WP_017382345.1 NZ_CP008823:c2421362-2420877 [Enterobacter cloacae ECNIH2]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARAFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T47627 NZ_CP008823:c2421362-2420877 [Enterobacter cloacae ECNIH2]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAGCGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGAGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT47627 WP_003857131.1 NZ_CP008823:c2421616-2421350 [Enterobacter cloacae ECNIH2]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT47627 NZ_CP008823:c2421616-2421350 [Enterobacter cloacae ECNIH2]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822W909

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References