Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1261254..1261874 Replicon chromosome
Accession NZ_CP008823
Organism UNVERIFIED_ORG: Enterobacter cloacae ECNIH2

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag ECNIH2_RS06085 Protein ID WP_015571250.1
Coordinates 1261254..1261472 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag ECNIH2_RS06090 Protein ID WP_006809850.1
Coordinates 1261500..1261874 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH2_RS06055 (ECNIH2_06055) 1257266..1257526 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
ECNIH2_RS06060 (ECNIH2_06060) 1257529..1257669 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
ECNIH2_RS06065 (ECNIH2_06065) 1257666..1258376 - 711 WP_023303201.1 GNAT family protein -
ECNIH2_RS06070 (ECNIH2_06070) 1258478..1259938 + 1461 WP_032620143.1 PLP-dependent aminotransferase family protein -
ECNIH2_RS06075 (ECNIH2_06075) 1259910..1260377 - 468 WP_017383209.1 YlaC family protein -
ECNIH2_RS06080 (ECNIH2_06080) 1260494..1261045 - 552 WP_023303203.1 maltose O-acetyltransferase -
ECNIH2_RS06085 (ECNIH2_06085) 1261254..1261472 - 219 WP_015571250.1 HHA domain-containing protein Toxin
ECNIH2_RS06090 (ECNIH2_06090) 1261500..1261874 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
ECNIH2_RS06095 (ECNIH2_06095) 1262385..1265531 - 3147 WP_044488974.1 multidrug efflux RND transporter permease subunit AcrB -
ECNIH2_RS06100 (ECNIH2_06100) 1265554..1266747 - 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T47626 WP_015571250.1 NZ_CP008823:c1261472-1261254 [Enterobacter cloacae ECNIH2]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T47626 NZ_CP008823:c1261472-1261254 [Enterobacter cloacae ECNIH2]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT47626 WP_006809850.1 NZ_CP008823:c1261874-1261500 [Enterobacter cloacae ECNIH2]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT47626 NZ_CP008823:c1261874-1261500 [Enterobacter cloacae ECNIH2]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References