Detailed information of TA system TA047622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2375964..2376661
• Replicon: chromosome
• Accession: NZ_CP008820
• Organism: Brucella anthropi strain OAB

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DR92_RS21475
• Protein ID: WP_040130219.1
• Coordinates: 2375964..2376389 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A256H0E6
• Locus tag: DR92_RS21480
• Protein ID: WP_012090891.1
• Coordinates: 2376389..2376661 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR92_RS21450	2371186..2372055	+	870	WP_049768389.1	carbohydrate ABC transporter permease	-
DR92_RS21455	2372145..2373374	+	1230	WP_115179296.1	ABC transporter substrate-binding protein	-
DR92_RS21460	2373567..2374874	-	1308	WP_040129727.1	IS1380 family transposase	-
DR92_RS21465	2375215..2375496	+	282	WP_041544966.1	hypothetical protein	-
DR92_RS21470	2375645..2375953	+	309	WP_012090893.1	HigA family addiction module antidote protein	-
DR92_RS21475	2375964..2376389	-	426	WP_040130219.1	type II toxin-antitoxin system VapC family toxin	Toxin
DR92_RS21480	2376389..2376661	-	273	WP_012090891.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DR92_RS21490	2377361..2378296	-	936	WP_012090889.1	isoaspartyl peptidase/L-asparaginase	-
DR92_RS21495	2378339..2379157	-	819	WP_012090888.1	M55 family metallopeptidase	-
DR92_RS21500	2379170..2380054	-	885	WP_012090887.1	ABC transporter permease subunit	-
DR92_RS21505	2380051..2380971	-	921	WP_012090886.1	glutathione ABC transporter permease GsiC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2373567..2374874	1307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (1-79)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 14970.04 Da
• Isoelectric Point: 4.4540

>T47622 WP_040130219.1 NZ_CP008820:c2376389-2375964 [Brucella anthropi]
MIVDASAIIAILFDEVEAPECMDALQSDTARLISAVNYVEAGTVLAGRIREGDRHAAIADLDAFLADFGILVASIDDRLA
RAAMKARVEYGKGFGTRAGLNFGDCFAYALAKRHSAPLLYVGDDFALTDIQSALSRGPCQG

Nucleotide

• Length: 426 bp

>T47622 NZ_CP008820:c2376389-2375964 [Brucella anthropi]
ATGATTGTCGACGCATCAGCGATCATAGCGATCCTCTTCGATGAGGTGGAAGCGCCGGAGTGCATGGATGCTCTCCAGTC
TGATACGGCGCGTCTGATATCGGCGGTGAATTACGTCGAGGCCGGTACAGTTCTTGCGGGCAGGATCAGGGAGGGAGATC
GGCATGCTGCGATTGCCGATCTGGATGCATTCCTTGCTGATTTCGGCATCTTGGTTGCGTCCATAGATGACAGGCTTGCA
CGAGCTGCGATGAAGGCGCGGGTGGAATACGGCAAGGGATTTGGAACGAGAGCCGGTTTGAACTTCGGTGATTGTTTTGC
CTATGCCTTGGCAAAACGGCATTCCGCTCCCCTTCTCTATGTCGGTGATGACTTTGCGCTGACTGACATTCAATCCGCGC
TATCACGCGGCCCCTGCCAAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10588.79 Da
• Isoelectric Point: 5.9303

>AT47622 WP_012090891.1 NZ_CP008820:c2376661-2376389 [Brucella anthropi]
MALSIKTDEADRLARELSRLTGETMTDAITQAMRERLDRLRAEREAQRDYVARMKAFVRERASRYDRRPVTKQEWDEAVG
DTSEEPGYHQ

Nucleotide

• Length: 273 bp

>AT47622 NZ_CP008820:c2376661-2376389 [Brucella anthropi]
ATGGCCTTGAGCATCAAGACCGATGAAGCAGACCGGTTGGCGCGGGAACTATCCCGTCTTACCGGCGAGACCATGACTGA
CGCTATCACTCAGGCCATGCGAGAGCGACTGGACCGGCTTCGTGCCGAGCGCGAGGCGCAGCGCGATTACGTTGCGCGTA
TGAAAGCTTTCGTTCGTGAGCGTGCCAGTCGTTATGACCGCCGTCCGGTTACGAAACAGGAATGGGACGAAGCCGTCGGC
GATACCTCAGAAGAGCCAGGCTATCATCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A256H0E6