Detailed information of TA system TA047620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 37852..38471
• Replicon: plasmid 1
• Accession: NZ_CP008817
• Organism: Brucella anthropi strain OAB

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A6I0DKZ7
• Locus tag: DR92_RS00220
• Protein ID: WP_038595052.1
• Coordinates: 37852..38148 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: DR92_RS00225
• Protein ID: WP_038595053.1
• Coordinates: 38154..38471 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR92_RS00185	33167..33418	-	252	WP_038595038.1	hypothetical protein	-
DR92_RS00190	33423..34691	-	1269	WP_038595040.1	type VI secretion protein ImpB	-
DR92_RS00195	34926..35114	-	189	WP_038595042.1	hypothetical protein	-
DR92_RS00200	35559..35873	+	315	WP_038595044.1	DUF736 domain-containing protein	-
DR92_RS00210	36714..37553	+	840	WP_038595048.1	methyltransferase	-
DR92_RS00215	37565..37807	+	243	WP_038595050.1	hypothetical protein	-
DR92_RS00220	37852..38148	+	297	WP_038595052.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DR92_RS00225	38154..38471	+	318	WP_038595053.1	putative addiction module antidote protein	Antitoxin
DR92_RS00230	38748..39044	+	297	WP_038595055.1	helix-turn-helix domain-containing protein	-
DR92_RS00235	39071..39355	+	285	WP_038595057.1	helix-turn-helix domain-containing protein	-
DR92_RS00240	39469..39666	+	198	WP_038595059.1	hypothetical protein	-
DR92_RS00245	39848..40030	+	183	WP_038595061.1	hypothetical protein	-
DR92_RS00250	40457..40729	+	273	WP_038595063.1	hypothetical protein	-
DR92_RS00255	40758..42539	-	1782	WP_038595065.1	type IV secretion system DNA-binding domain-containing protein	-
DR92_RS00260	42532..42729	-	198	WP_038595068.1	conjugal transfer protein TraD	-
DR92_RS00265	42779..42988	-	210	WP_038595237.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..155838	155838

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (4-100)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11022.80 Da
• Isoelectric Point: 10.5350

>T47620 WP_038595052.1 NZ_CP008817:37852-38148 [Brucella anthropi]
MIELIETDIFSRWIGALRDRQARARIQARMRRLSLGNPGDVKPVGEGISEMRIDHGPGYRVYFMRRGPVIVVLLCGGDKG
SQSRDIALAKDIAGQWKE

Nucleotide

• Length: 297 bp

>T47620 NZ_CP008817:37852-38148 [Brucella anthropi]
ATGATCGAACTCATTGAAACCGACATTTTTTCCCGCTGGATTGGGGCACTGCGCGACCGTCAGGCTCGGGCAAGGATTCA
AGCCCGGATGCGTCGATTAAGCCTTGGAAATCCCGGCGATGTTAAGCCGGTAGGCGAAGGCATTTCCGAAATGAGGATCG
ACCATGGACCCGGTTATCGGGTCTATTTCATGCGGCGCGGCCCTGTAATTGTGGTGCTTCTTTGCGGAGGCGACAAGGGA
AGTCAGAGCAGGGATATAGCCTTGGCAAAGGACATTGCAGGGCAATGGAAGGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11219.82 Da
• Isoelectric Point: 5.8294

>AT47620 WP_038595053.1 NZ_CP008817:38154-38471 [Brucella anthropi]
MEVQFRRYDAANYLRTEDDIAAYIEAVMEDGDPALIAAALGDVARARNLSQLAREVGMSRQGLDKALSGNGNPSLATVMK
VANALGLRLSLKPIHTGEPTPALKP

Nucleotide

• Length: 318 bp

>AT47620 NZ_CP008817:38154-38471 [Brucella anthropi]
ATGGAAGTTCAATTCAGACGCTATGACGCGGCAAACTATCTCAGAACAGAAGACGACATTGCGGCTTATATTGAAGCCGT
GATGGAGGATGGAGATCCTGCATTAATCGCAGCCGCGCTTGGAGACGTTGCCCGCGCTCGCAATCTAAGCCAGCTTGCCC
GCGAAGTCGGAATGAGCCGCCAAGGGCTGGACAAAGCGCTATCGGGCAATGGTAATCCAAGTCTGGCAACTGTAATGAAG
GTCGCAAACGCTCTTGGTTTAAGACTTTCGTTGAAGCCAATTCATACCGGCGAACCAACTCCGGCCCTCAAACCATGA

Structures

Toxin

• AlphaFold DB: A0A6I0DKZ7