Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 32239..32883 Replicon plasmid pSS17
Accession NZ_CP008807
Organism Escherichia coli O157:H7 str. SS17

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag SS17_RS29200 Protein ID WP_000833471.1
Coordinates 32701..32883 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag SS17_RS29195 Protein ID WP_000466317.1
Coordinates 32239..32676 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SS17_RS29175 29650..29985 - 336 WP_000963830.1 hypothetical protein -
SS17_RS29180 30331..30738 + 408 WP_001190619.1 hypothetical protein -
SS17_RS29185 30748..31509 + 762 WP_001097937.1 conjugal transfer protein TraL -
SS17_RS29190 31506..32210 + 705 WP_001279868.1 hypothetical protein -
SS17_RS29195 32239..32676 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SS17_RS29200 32701..32883 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
SS17_RS29205 33311..33589 - 279 WP_000545934.1 hypothetical protein -
SS17_RS29210 33647..33976 - 330 WP_000449469.1 hypothetical protein -
SS17_RS29215 34416..34658 - 243 WP_001450803.1 hypothetical protein -
SS17_RS29220 34648..34914 - 267 WP_040126099.1 hypothetical protein -
SS17_RS29225 34965..35189 - 225 WP_000749827.1 hypothetical protein -
SS17_RS29230 35285..35509 - 225 WP_000866040.1 hypothetical protein -
SS17_RS29235 35549..35872 - 324 WP_000432877.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37447 37447


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T47579 WP_000833471.1 NZ_CP008807:c32883-32701 [Escherichia coli O157:H7 str. SS17]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T47579 NZ_CP008807:c32883-32701 [Escherichia coli O157:H7 str. SS17]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT47579 WP_000466317.1 NZ_CP008807:c32676-32239 [Escherichia coli O157:H7 str. SS17]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT47579 NZ_CP008807:c32676-32239 [Escherichia coli O157:H7 str. SS17]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References