Detailed information of TA system TA047578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 57869..58428
• Replicon: plasmid p0157
• Accession: NZ_CP008806
• Organism: Escherichia coli O157:H7 str. SS17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: SS17_RS28830
• Protein ID: WP_000421248.1
• Coordinates: 58153..58428 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: SS17_RS28825
• Protein ID: WP_001178089.1
• Coordinates: 57869..58153 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS17_RS28785	53060..53620	+	561	WP_000840472.1	fertility inhibition protein FinO	-
SS17_RS28790	53753..53965	+	213	WP_001302189.1	ANR family transcriptional regulator	-
SS17_RS28795	54210..54671	+	462	WP_001233856.1	thermonuclease family protein	-
SS17_RS28800	54717..54926	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
SS17_RS28805	54964..55302	+	339	WP_000766796.1	hypothetical protein	-
SS17_RS28810	55542..55796	+	255	WP_000083831.1	replication regulatory protein RepA	-
SS17_RS28815	56032..56106	+	75	WP_001370046.1	RepA leader peptide Tap	-
SS17_RS28820	56099..56956	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
SS17_RS28825	57869..58153	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
SS17_RS28830	58153..58428	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SS17_RS28835	58523..58729	+	207	WP_001105064.1	hypothetical protein	-
SS17_RS28840	58829..59044	-	216	Protein_64	transposase	-
SS17_RS31840	59088..59654	-	567	Protein_65	IS3-like element IS600 family transposase	-
SS17_RS28850	59654..60070	-	417	Protein_66	IS91 family transposase	-
SS17_RS28855	60070..60255	-	186	WP_001302179.1	hypothetical protein	-
SS17_RS28860	60432..62642	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
SS17_RS28865	62686..63075	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE / exeE / exeG	1..94645	94645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T47578 WP_000421248.1 NZ_CP008806:58153-58428 [Escherichia coli O157:H7 str. SS17]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T47578 NZ_CP008806:58153-58428 [Escherichia coli O157:H7 str. SS17]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT47578 WP_001178089.1 NZ_CP008806:57869-58153 [Escherichia coli O157:H7 str. SS17]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT47578 NZ_CP008806:57869-58153 [Escherichia coli O157:H7 str. SS17]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8