Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4414333..4414964 Replicon chromosome
Accession NZ_CP008805
Organism Escherichia coli O157:H7 str. SS17

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag SS17_RS23110 Protein ID WP_001259385.1
Coordinates 4414689..4414964 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag SS17_RS23105 Protein ID WP_000593555.1
Coordinates 4414333..4414692 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SS17_RS23075 4409445..4409918 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
SS17_RS23080 4409915..4410196 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
SS17_RS23085 4410273..4411631 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
SS17_RS23090 4411624..4413132 + 1509 WP_001301773.1 carbohydrate kinase -
SS17_RS23095 4413122..4413391 + 270 WP_000933060.1 HPr family phosphocarrier protein -
SS17_RS23100 4413423..4414283 + 861 WP_001131788.1 class II aldolase -
SS17_RS23105 4414333..4414692 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SS17_RS23110 4414689..4414964 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
SS17_RS23115 4415037..4416161 - 1125 WP_001301659.1 ABC transporter permease -
SS17_RS23120 4416161..4418896 - 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
SS17_RS23125 4418893..4419960 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T47569 WP_001259385.1 NZ_CP008805:c4414964-4414689 [Escherichia coli O157:H7 str. SS17]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T47569 NZ_CP008805:c4414964-4414689 [Escherichia coli O157:H7 str. SS17]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT47569 WP_000593555.1 NZ_CP008805:c4414692-4414333 [Escherichia coli O157:H7 str. SS17]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT47569 NZ_CP008805:c4414692-4414333 [Escherichia coli O157:H7 str. SS17]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References