Detailed information of TA system TA047553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2152111..2152582
• Replicon: chromosome
• Accession: NZ_CP008805
• Organism: Escherichia coli O157:H7 str. SS17

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: SS17_RS11110
• Protein ID: WP_001303511.1
• Coordinates: 2152304..2152582 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: SS17_RS11105
• Protein ID: WP_001302048.1
• Coordinates: 2152111..2152302 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS17_RS11060	2147318..2147902	-	585	WP_000206793.1	DUF551 domain-containing protein	-
SS17_RS11065	2147959..2148354	-	396	WP_001118159.1	DUF977 family protein	-
SS17_RS11070	2148370..2149139	-	770	Protein_2103	DUF1627 domain-containing protein	-
SS17_RS11080	2149165..2149905	-	741	WP_000788938.1	ATP-binding protein	-
SS17_RS11085	2149912..2150874	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
SS17_RS11090	2150897..2151322	-	426	WP_000693943.1	Rha family transcriptional regulator	-
SS17_RS11095	2151319..2151621	-	303	WP_000172738.1	transcriptional regulator	-
SS17_RS32310	2151719..2152090	+	372	WP_001169686.1	hypothetical protein	-
SS17_RS11105	2152111..2152302	+	192	WP_001302048.1	hypothetical protein	Antitoxin
SS17_RS11110	2152304..2152582	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SS17_RS11120	2152933..2153232	+	300	WP_001240334.1	hypothetical protein	-
SS17_RS11125	2153304..2153522	+	219	WP_001171930.1	hypothetical protein	-
SS17_RS30450	2154091..2154279	+	189	WP_000449192.1	cell division inhibition protein DicB	-
SS17_RS11130	2154276..2154467	+	192	WP_001090200.1	DUF1482 family protein	-
SS17_RS11135	2154560..2155645	+	1086	Protein_2115	exonuclease	-
SS17_RS11145	2155696..2156909	+	1214	WP_162829202.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2069681..2168773	99092

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T47553 WP_001303511.1 NZ_CP008805:2152304-2152582 [Escherichia coli O157:H7 str. SS17]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T47553 NZ_CP008805:2152304-2152582 [Escherichia coli O157:H7 str. SS17]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT47553 WP_001302048.1 NZ_CP008805:2152111-2152302 [Escherichia coli O157:H7 str. SS17]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT47553 NZ_CP008805:2152111-2152302 [Escherichia coli O157:H7 str. SS17]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0