Detailed information of TA system TA047542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1169810..1170288
• Replicon: chromosome
• Accession: NZ_CP008805
• Organism: Escherichia coli O157:H7 str. SS17

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: SS17_RS05570
• Protein ID: WP_001303876.1
• Coordinates: 1169810..1170097 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: SS17_RS05575
• Protein ID: WP_000536233.1
• Coordinates: 1170097..1170288 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS17_RS05535	1164961..1167429	-	2469	WP_038430871.1	exonuclease	-
SS17_RS05540	1167523..1167714	-	192	WP_001098307.1	DUF1482 family protein	-
SS17_RS05545	1167711..1167899	-	189	WP_000413705.1	cell division inhibition protein DicB	-
SS17_RS05555	1168475..1168660	+	186	WP_001133046.1	hypothetical protein	-
SS17_RS05560	1168847..1169236	-	390	WP_000394511.1	hypothetical protein	-
SS17_RS05565	1169378..1169533	-	156	WP_000379575.1	DUF1391 family protein	-
SS17_RS05570	1169810..1170097	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SS17_RS05575	1170097..1170288	-	192	WP_000536233.1	hypothetical protein	Antitoxin
SS17_RS05580	1170316..1170717	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
SS17_RS05585	1170825..1171097	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
SS17_RS05590	1171081..1171506	+	426	WP_000693855.1	Rha family transcriptional regulator	-
SS17_RS32005	1171713..1172168	-	456	WP_000273724.1	hypothetical protein	-
SS17_RS05600	1172247..1173338	+	1092	WP_001205823.1	hypothetical protein	-
SS17_RS05605	1173345..1174091	+	747	WP_000788745.1	ATP-binding protein	-
SS17_RS05610	1174113..1174883	+	771	WP_000450992.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T47542 WP_001303876.1 NZ_CP008805:c1170097-1169810 [Escherichia coli O157:H7 str. SS17]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T47542 NZ_CP008805:c1170097-1169810 [Escherichia coli O157:H7 str. SS17]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT47542 WP_000536233.1 NZ_CP008805:c1170288-1170097 [Escherichia coli O157:H7 str. SS17]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT47542 NZ_CP008805:c1170288-1170097 [Escherichia coli O157:H7 str. SS17]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA