Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3002897..3003422 Replicon chromosome
Accession NZ_CP008801
Organism Escherichia coli KLY

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HQ24_RS14865 Protein ID WP_001159868.1
Coordinates 3003117..3003422 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HQ24_RS14860 Protein ID WP_000813634.1
Coordinates 3002897..3003115 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQ24_RS14845 2998053..2998952 + 900 WP_000963206.1 nucleotide-binding protein -
HQ24_RS14850 2999002..3001227 - 2226 WP_000698737.1 phage T7 exclusion protein -
HQ24_RS14855 3001229..3002317 - 1089 WP_000952217.1 transcriptional repressor PifC -
HQ24_RS14860 3002897..3003115 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HQ24_RS14865 3003117..3003422 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HQ24_RS14870 3003423..3004229 + 807 WP_000016982.1 site-specific integrase -
HQ24_RS14875 3005003..3005758 + 756 WP_000852146.1 replication initiation protein RepE -
HQ24_RS14880 3006346..3007512 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2974230..3074293 100063


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T47517 WP_001159868.1 NZ_CP008801:3003117-3003422 [Escherichia coli KLY]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T47517 NZ_CP008801:3003117-3003422 [Escherichia coli KLY]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT47517 WP_000813634.1 NZ_CP008801:3002897-3003115 [Escherichia coli KLY]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT47517 NZ_CP008801:3002897-3003115 [Escherichia coli KLY]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References