Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2052817..2053648 Replicon chromosome
Accession NZ_CP008801
Organism Escherichia coli KLY

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HQ24_RS10325 Protein ID WP_000854814.1
Coordinates 2053274..2053648 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag HQ24_RS10320 Protein ID WP_001285584.1
Coordinates 2052817..2053185 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQ24_RS10305 2050484..2052016 + 1533 WP_001350525.1 hypothetical protein -
HQ24_RS25715 2052013..2052459 + 447 WP_000187523.1 RadC family protein -
HQ24_RS10315 2052522..2052743 + 222 WP_000692323.1 DUF987 domain-containing protein -
HQ24_RS10320 2052817..2053185 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
HQ24_RS10325 2053274..2053648 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
HQ24_RS10330 2053645..2053839 + 195 WP_000988600.1 hypothetical protein -
HQ24_RS26540 2053852..2053965 + 114 WP_001161660.1 DUF957 domain-containing protein -
HQ24_RS24465 2054254..2054382 - 129 Protein_1971 transposase domain-containing protein -
HQ24_RS10340 2054454..2054636 + 183 WP_001016348.1 hypothetical protein -
HQ24_RS10345 2054737..2055066 - 330 WP_000450409.1 DUF496 family protein -
HQ24_RS10350 2055238..2056296 - 1059 WP_001200891.1 FUSC family protein -
HQ24_RS10355 2056494..2056967 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
HQ24_RS10360 2057086..2058252 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T47512 WP_000854814.1 NZ_CP008801:2053274-2053648 [Escherichia coli KLY]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T47512 NZ_CP008801:2053274-2053648 [Escherichia coli KLY]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT47512 WP_001285584.1 NZ_CP008801:2052817-2053185 [Escherichia coli KLY]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT47512 NZ_CP008801:2052817-2053185 [Escherichia coli KLY]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References