Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 71279..72015 Replicon plasmid pKPN-e44
Accession NZ_CP008800
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH24

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KPNIH24_RS28410 Protein ID WP_003026803.1
Coordinates 71279..71761 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KPNIH24_RS28415 Protein ID WP_003026799.1
Coordinates 71749..72015 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH24_RS28390 66916..67878 + 963 WP_004152113.1 M48 family metalloprotease -
KPNIH24_RS31720 67966..69220 + 1255 Protein_65 IS3 family transposase -
KPNIH24_RS28405 69721..71067 - 1347 WP_020314316.1 ISNCY family transposase -
KPNIH24_RS28410 71279..71761 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KPNIH24_RS28415 71749..72015 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KPNIH24_RS28420 72191..72445 - 255 WP_004152108.1 hypothetical protein -
KPNIH24_RS28425 72521..72778 - 258 WP_004152107.1 hypothetical protein -
KPNIH24_RS28430 72827..73030 - 204 WP_004152106.1 hemolysin expression modulator Hha -
KPNIH24_RS28435 73064..73432 - 369 WP_004152105.1 hypothetical protein -
KPNIH24_RS28440 73476..73970 - 495 WP_004152104.1 DNA-binding protein -
KPNIH24_RS28445 74001..74576 - 576 WP_004152103.1 hypothetical protein -
KPNIH24_RS28450 74564..74833 - 270 WP_004152102.1 hypothetical protein -
KPNIH24_RS28455 75191..75541 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KPNIH24_RS28460 75591..75953 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia - 1..194877 194877


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T47486 WP_003026803.1 NZ_CP008800:c71761-71279 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T47486 NZ_CP008800:c71761-71279 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT47486 WP_003026799.1 NZ_CP008800:c72015-71749 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT47486 NZ_CP008800:c72015-71749 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References