Detailed information of TA system TA047485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 54142..54785
• Replicon: plasmid pKPC-484
• Accession: NZ_CP008798
• Organism: Klebsiella pneumoniae subsp. pneumoniae KPNIH24

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: KPNIH24_RS27575
• Protein ID: WP_001044770.1
• Coordinates: 54142..54558 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: KPNIH24_RS27580
• Protein ID: WP_001261282.1
• Coordinates: 54555..54785 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPNIH24_RS27535	49684..49920	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
KPNIH24_RS27540	49917..50279	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
KPNIH24_RS27545	50297..51991	-	1695	WP_000105636.1	mercury(II) reductase	-
KPNIH24_RS27550	52043..52465	-	423	WP_001340589.1	organomercurial transporter MerC	-
KPNIH24_RS27555	52501..52626	-	126	WP_072093211.1	mercury transporter	-
KPNIH24_RS27560	52627..53324	-	698	Protein_58	IS1 family transposase	-
KPNIH24_RS27570	53358..54068	-	711	WP_004152334.1	ATP-binding protein	-
KPNIH24_RS27575	54142..54558	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
KPNIH24_RS27580	54555..54785	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KPNIH24_RS27585	54742..55203	+	462	WP_072202616.1	hypothetical protein	-
KPNIH24_RS27590	55347..55697	+	351	WP_000493378.1	hypothetical protein	-
KPNIH24_RS27595	55748..56491	+	744	WP_000129823.1	hypothetical protein	-
KPNIH24_RS27600	56488..57264	+	777	WP_000015958.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / sul3 / aac(3)-IVa / aph(4)-Ia / blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / dfrA12 / aadA2 / qacE / sul1	-	1..85473	85473
	-	inside	IScluster/Tn	aac(6')-Ib	-	52627..63103	10476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T47485 WP_001044770.1 NZ_CP008798:c54558-54142 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T47485 NZ_CP008798:c54558-54142 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT47485 WP_001261282.1 NZ_CP008798:c54785-54555 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT47485 NZ_CP008798:c54785-54555 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3