Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4670690..4671309 Replicon chromosome
Accession NZ_CP008797
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH24

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KPNIH24_RS23620 Protein ID WP_002892050.1
Coordinates 4671091..4671309 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KPNIH24_RS23615 Protein ID WP_002892066.1
Coordinates 4670690..4671064 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH24_RS23605 4665842..4667035 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KPNIH24_RS23610 4667058..4670204 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KPNIH24_RS23615 4670690..4671064 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KPNIH24_RS23620 4671091..4671309 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KPNIH24_RS23625 4671468..4672034 + 567 WP_002892030.1 maltose O-acetyltransferase -
KPNIH24_RS32275 4672006..4672146 - 141 WP_004147370.1 hypothetical protein -
KPNIH24_RS23635 4672167..4672637 + 471 WP_002892026.1 YlaC family protein -
KPNIH24_RS23640 4672612..4674063 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KPNIH24_RS23645 4674164..4674862 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
KPNIH24_RS23650 4674859..4674999 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KPNIH24_RS23655 4674999..4675262 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T47482 WP_002892050.1 NZ_CP008797:4671091-4671309 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T47482 NZ_CP008797:4671091-4671309 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT47482 WP_002892066.1 NZ_CP008797:4670690-4671064 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT47482 NZ_CP008797:4670690-4671064 [Klebsiella pneumoniae subsp. pneumoniae KPNIH24]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References