Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 30860..31596 Replicon plasmid pKOX-137
Accession NZ_CP008789
Organism Klebsiella oxytoca KONIH1

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag KONIH1_RS28985 Protein ID WP_004098919.1
Coordinates 31114..31596 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag KONIH1_RS28980 Protein ID WP_004098921.1
Coordinates 30860..31126 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KONIH1_RS28935 26043..27044 + 1002 WP_009654304.1 Abi family protein -
KONIH1_RS28940 27183..27761 - 579 WP_009654302.1 hypothetical protein -
KONIH1_RS28945 28051..28314 + 264 WP_009310051.1 hypothetical protein -
KONIH1_RS28950 28311..28877 + 567 WP_009654312.1 hypothetical protein -
KONIH1_RS28955 28908..29402 + 495 WP_013023776.1 hypothetical protein -
KONIH1_RS28960 29446..29814 + 369 WP_042946273.1 hypothetical protein -
KONIH1_RS28965 29845..30048 + 204 WP_004098931.1 hemolysin expression modulator Hha -
KONIH1_RS28970 30097..30354 + 258 WP_004098928.1 hypothetical protein -
KONIH1_RS28975 30430..30684 + 255 WP_004118702.1 hypothetical protein -
KONIH1_RS28980 30860..31126 + 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
KONIH1_RS28985 31114..31596 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
KONIH1_RS28990 31805..33151 + 1347 WP_077255298.1 ISNCY family transposase -
KONIH1_RS28995 33210..34001 + 792 WP_003032641.1 tetratricopeptide repeat protein -
KONIH1_RS29000 34042..34824 - 783 Protein_38 hypothetical protein -
KONIH1_RS34835 34814..34969 + 156 Protein_39 IS3 family transposase -
KONIH1_RS29005 35033..35737 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KONIH1_RS29010 35863..36153 + 291 WP_001247892.1 nucleotidyltransferase -
KONIH1_RS29015 36150..36551 + 402 WP_001293886.1 DUF86 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..133397 133397


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T47461 WP_004098919.1 NZ_CP008789:31114-31596 [Klebsiella oxytoca KONIH1]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T47461 NZ_CP008789:31114-31596 [Klebsiella oxytoca KONIH1]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT47461 WP_004098921.1 NZ_CP008789:30860-31126 [Klebsiella oxytoca KONIH1]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT47461 NZ_CP008789:30860-31126 [Klebsiella oxytoca KONIH1]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTAGAGATTGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References