Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 6030542..6031089 Replicon chromosome
Accession NZ_CP008788
Organism Klebsiella oxytoca KONIH1

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag KONIH1_RS28225 Protein ID WP_014227299.1
Coordinates 6030781..6031089 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag KONIH1_RS28220 Protein ID WP_014227298.1
Coordinates 6030542..6030778 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KONIH1_RS28205 6026749..6027915 + 1167 WP_042946137.1 multidrug effflux MFS transporter -
KONIH1_RS28210 6028076..6028978 + 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
KONIH1_RS28215 6029003..6030436 + 1434 WP_032718833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
KONIH1_RS28220 6030542..6030778 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KONIH1_RS28225 6030781..6031089 + 309 WP_014227299.1 CcdB family protein Toxin
KONIH1_RS28230 6031240..6032142 + 903 WP_014227300.1 EamA family transporter -
KONIH1_RS28235 6032146..6032403 - 258 WP_042946138.1 hypothetical protein -
KONIH1_RS28240 6032403..6033368 - 966 WP_038423881.1 Rpn family recombination-promoting nuclease/putative transposase -
KONIH1_RS28245 6033578..6033871 + 294 WP_014227302.1 hypothetical protein -
KONIH1_RS28250 6033895..6034836 - 942 WP_014227303.1 LacI family transcriptional regulator -
KONIH1_RS28255 6035131..6035442 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T47459 WP_014227299.1 NZ_CP008788:6030781-6031089 [Klebsiella oxytoca KONIH1]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T47459 NZ_CP008788:6030781-6031089 [Klebsiella oxytoca KONIH1]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGTATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT47459 WP_014227298.1 NZ_CP008788:6030542-6030778 [Klebsiella oxytoca KONIH1]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT47459 NZ_CP008788:6030542-6030778 [Klebsiella oxytoca KONIH1]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References