Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1460945..1461564 Replicon chromosome
Accession NZ_CP008788
Organism Klebsiella oxytoca KONIH1

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag KONIH1_RS06805 Protein ID WP_004099646.1
Coordinates 1460945..1461163 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag KONIH1_RS06810 Protein ID WP_004129911.1
Coordinates 1461190..1461564 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KONIH1_RS06775 1456990..1457253 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
KONIH1_RS06780 1457253..1457393 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KONIH1_RS06785 1457390..1458088 - 699 WP_014228203.1 GNAT family N-acetyltransferase -
KONIH1_RS06790 1458190..1459644 + 1455 WP_042944459.1 PLP-dependent aminotransferase family protein -
KONIH1_RS06795 1459619..1460089 - 471 WP_014228205.1 YlaC family protein -
KONIH1_RS06800 1460216..1460782 - 567 WP_042944461.1 maltose O-acetyltransferase -
KONIH1_RS06805 1460945..1461163 - 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
KONIH1_RS06810 1461190..1461564 - 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
KONIH1_RS06815 1462045..1465191 - 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
KONIH1_RS06820 1465214..1466407 - 1194 WP_042944463.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T47447 WP_004099646.1 NZ_CP008788:c1461163-1460945 [Klebsiella oxytoca KONIH1]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T47447 NZ_CP008788:c1461163-1460945 [Klebsiella oxytoca KONIH1]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT47447 WP_004129911.1 NZ_CP008788:c1461564-1461190 [Klebsiella oxytoca KONIH1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT47447 NZ_CP008788:c1461564-1461190 [Klebsiella oxytoca KONIH1]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References