Detailed information of TA system TA047444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 739198..739774
• Replicon: chromosome
• Accession: NZ_CP008788
• Organism: Klebsiella oxytoca KONIH1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: KONIH1_RS03480
• Protein ID: WP_038424021.1
• Coordinates: 739198..739485 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: KONIH1_RS03485
• Protein ID: WP_014227757.1
• Coordinates: 739472..739774 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KONIH1_RS03450	734540..735058	-	519	WP_042944214.1	hypothetical protein	-
KONIH1_RS03455	735055..735855	-	801	WP_014227752.1	winged helix-turn-helix domain-containing protein	-
KONIH1_RS03460	736001..736585	-	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
KONIH1_RS03465	736733..737515	-	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
KONIH1_RS03470	737603..738511	+	909	WP_014837311.1	LysR family transcriptional regulator	-
KONIH1_RS03475	738505..738948	+	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
KONIH1_RS03480	739198..739485	+	288	WP_038424021.1	BrnT family toxin	Toxin
KONIH1_RS03485	739472..739774	+	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
KONIH1_RS03490	739895..741238	-	1344	WP_032719935.1	APC family permease	-
KONIH1_RS03495	741235..742146	-	912	WP_032719936.1	acetamidase/formamidase family protein	-
KONIH1_RS03500	742599..742934	-	336	WP_014227760.1	endoribonuclease SymE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11253.72 Da
• Isoelectric Point: 8.5899

>T47444 WP_038424021.1 NZ_CP008788:739198-739485 [Klebsiella oxytoca KONIH1]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T47444 NZ_CP008788:739198-739485 [Klebsiella oxytoca KONIH1]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAGGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT47444 WP_014227757.1 NZ_CP008788:739472-739774 [Klebsiella oxytoca KONIH1]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT47444 NZ_CP008788:739472-739774 [Klebsiella oxytoca KONIH1]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591