Detailed information of TA system TA047443

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 682946..683717
• Replicon: chromosome
• Accession: NZ_CP008788
• Organism: Klebsiella oxytoca KONIH1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2J4ZCW3
• Locus tag: KONIH1_RS03220
• Protein ID: WP_042944193.1
• Coordinates: 683328..683717 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KONIH1_RS03215
• Protein ID: WP_171840905.1
• Coordinates: 682946..683272 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KONIH1_RS32185	679386..680018	-	633	WP_049100008.1	inovirus Gp2 family protein	-
KONIH1_RS03190	680561..681385	+	825	WP_042944188.1	DUF945 domain-containing protein	-
KONIH1_RS03195	681391..681639	+	249	WP_049100007.1	DUF905 domain-containing protein	-
KONIH1_RS03200	681719..682162	+	444	WP_042944189.1	antirestriction protein	-
KONIH1_RS03205	682174..682653	+	480	WP_042944191.1	DNA repair protein RadC	-
KONIH1_RS03210	682667..682888	+	222	WP_004192297.1	DUF987 domain-containing protein	-
KONIH1_RS03215	682946..683272	+	327	WP_171840905.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KONIH1_RS03220	683328..683717	+	390	WP_042944193.1	TA system toxin CbtA family protein	Toxin
KONIH1_RS03225	683804..684649	+	846	WP_042944195.1	DUF4942 domain-containing protein	-
KONIH1_RS03230	684792..685589	+	798	WP_042944196.1	helix-turn-helix transcriptional regulator	-
KONIH1_RS03235	685767..686510	-	744	WP_014227721.1	hypothetical protein	-
KONIH1_RS03240	686676..687110	-	435	WP_004098173.1	VOC family protein	-
KONIH1_RS03245	687257..687580	-	324	WP_032747696.1	endoribonuclease SymE	-
KONIH1_RS03250	687870..688664	-	795	WP_038424010.1	DNA/RNA non-specific endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	643006..687580	44574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14418.54 Da
• Isoelectric Point: 8.9846

>T47443 WP_042944193.1 NZ_CP008788:683328-683717 [Klebsiella oxytoca KONIH1]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T47443 NZ_CP008788:683328-683717 [Klebsiella oxytoca KONIH1]
ATGCAAACAAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGTGATGAGCAGGTCATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGTTGATGCCCTGAATTTTATCGTTGAGAAGTATGAACTGGTCCGCACTGACCGCCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGGAAAGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGCGCTCCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12074.85 Da
• Isoelectric Point: 6.9367

>AT47443 WP_171840905.1 NZ_CP008788:682946-683272 [Klebsiella oxytoca KONIH1]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANVSGQFCDADLRHLDQAFPVLMKQLELMLTCGELNPRHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT47443 NZ_CP008788:682946-683272 [Klebsiella oxytoca KONIH1]
ATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTCGGTGCCCGTCTGGTCCAGGAGGGAAACCGCCT
GCATTACCTGGCTGACCGGGCGAATGTTAGCGGGCAGTTCTGTGATGCGGATTTACGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTCACCTGCGGTGAACTGAATCCCCGTCATCAGCACTGCGTCACGTTGTATGCA
AAAGGTCTGACCTGCGAGGCTGATACGCTGGGTTCGCACGGCTATGTTTATATCGCGATTTATCCCACTCCCGTCGCCAC
GGCATAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZCW3