Detailed information of TA system TA047442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1574739..1575276
• Replicon: chromosome
• Accession: NZ_CP008786
• Organism: Burkholderia thailandensis E264

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: DR63_RS26600
• Protein ID: WP_004549731.1
• Coordinates: 1575007..1575276 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q2T6G1
• Locus tag: DR63_RS26595
• Protein ID: WP_011401115.1
• Coordinates: 1574739..1575023 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR63_RS26570	1569764..1570606	-	843	WP_011401119.1	Clp protease ClpP	-
DR63_RS26575	1570584..1571879	-	1296	WP_009896509.1	phage portal protein	-
DR63_RS26580	1571887..1573629	-	1743	WP_011401117.1	terminase large subunit	-
DR63_RS26585	1573629..1574108	-	480	WP_025404174.1	terminase small subunit	-
DR63_RS26595	1574739..1575023	-	285	WP_011401115.1	BrnA antitoxin family protein	Antitoxin
DR63_RS26600	1575007..1575276	-	270	WP_004549731.1	BrnT family toxin	Toxin
DR63_RS26605	1575326..1575769	-	444	WP_009896499.1	hypothetical protein	-
DR63_RS26610	1575766..1576026	-	261	WP_009896498.1	hypothetical protein	-
DR63_RS26615	1576002..1576334	-	333	WP_011401114.1	hypothetical protein	-
DR63_RS26625	1576774..1577130	-	357	WP_009896497.1	hypothetical protein	-
DR63_RS26630	1577127..1577678	-	552	WP_011401112.1	DUF1064 domain-containing protein	-
DR63_RS26635	1577675..1578667	-	993	WP_015967393.1	hypothetical protein	-
DR63_RS26640	1578821..1579081	-	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
DR63_RS26645	1579078..1579899	-	822	WP_009896491.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1550065..1594708	44643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T47442 WP_004549731.1 NZ_CP008786:c1575276-1575007 [Burkholderia thailandensis E264]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T47442 NZ_CP008786:c1575276-1575007 [Burkholderia thailandensis E264]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10669.09 Da
• Isoelectric Point: 5.8930

>AT47442 WP_011401115.1 NZ_CP008786:c1575023-1574739 [Burkholderia thailandensis E264]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT47442 NZ_CP008786:c1575023-1574739 [Burkholderia thailandensis E264]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTACGACGTCGACATCGTGGACGCCTTCAAGGCAACAGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAAGGAGCACCAGCCTGCGTGA

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: Q2T6G1