Detailed information of TA system TA047440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 346372..347011
• Replicon: chromosome
• Accession: NZ_CP008786
• Organism: Burkholderia thailandensis E264

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q2T3Q6
• Locus tag: DR63_RS21295
• Protein ID: WP_009893919.1
• Coordinates: 346372..346551 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q2T3Q7
• Locus tag: DR63_RS21300
• Protein ID: WP_011401461.1
• Coordinates: 346595..347011 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR63_RS21270	341761..342741	+	981	WP_043037760.1	DUF3644 domain-containing protein	-
DR63_RS21275	343063..343638	+	576	WP_009893925.1	recombinase family protein	-
DR63_RS21280	343709..344824	-	1116	WP_009893924.1	DUF3396 domain-containing protein	-
DR63_RS21285	344834..345568	-	735	WP_011401463.1	VRR-NUC domain-containing protein	-
DR63_RS21290	345565..346092	-	528	WP_011401462.1	PAAR domain-containing protein	-
DR63_RS21295	346372..346551	+	180	WP_009893919.1	type II toxin-antitoxin system HicA family toxin	Toxin
DR63_RS21300	346595..347011	+	417	WP_011401461.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DR63_RS21310	347440..347937	-	498	WP_011401460.1	hypothetical protein	-
DR63_RS21315	348556..349128	-	573	WP_009893915.1	site-specific integrase	-
DR63_RS21320	349181..349915	-	735	WP_009893914.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	327441..347011	19570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6618.82 Da
• Isoelectric Point: 11.2336

>T47440 WP_009893919.1 NZ_CP008786:346372-346551 [Burkholderia thailandensis E264]
MNSSKLIRMLEEDGWRLVRVTGSHHHFKHPKKPGLVTVPHPKKDLPIGTVKSIQKSAGL

Nucleotide

• Length: 180 bp

>T47440 NZ_CP008786:346372-346551 [Burkholderia thailandensis E264]
ATGAACTCATCGAAGCTGATCCGGATGCTTGAGGAAGATGGCTGGAGGTTGGTTCGAGTGACAGGCAGCCATCATCATTT
CAAACACCCCAAGAAGCCAGGTCTTGTGACGGTTCCCCACCCGAAGAAGGACCTACCGATTGGGACTGTAAAAAGCATCC
AGAAATCCGCCGGCCTGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.03 Da
• Isoelectric Point: 4.6944

>AT47440 WP_011401461.1 NZ_CP008786:346595-347011 [Burkholderia thailandensis E264]
MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGDDVEFTCSTVEELVAKPEYAGAVWAL
VSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSGFLARAALEALNEGKVRHA

Nucleotide

• Length: 417 bp

>AT47440 NZ_CP008786:346595-347011 [Burkholderia thailandensis E264]
ATGGAATTTCCCATCGCAGTGCATAAGGACGATGGGAGCGTCTACGGCGTGACGGTCCCGGATATTCCGGGCGTTCACTC
CTGGGGCGAAACCATCGATGATGCAATCAAAAACACGAGAGAGGCTATCGTCGGCCATGTAGAGACATTGATCGAGCTTG
GGGACGATGTTGAATTCACTTGCTCGACGGTGGAAGAACTGGTTGCAAAGCCCGAGTACGCCGGGGCGGTTTGGGCGCTC
GTCAGCGTTGATCTTTCTCAGCTTGATTCCAAGCCCGAGCGGATCAATGTGAGTATCCCTCGCTTCGTGCTGCACAAGAT
TGACGCCTATGTGGCATCCCGCCACGAGACTAGAAGCGGATTTCTTGCGCGTGCTGCCCTGGAGGCACTTAATGAAGGCA
AAGTTAGGCACGCATAA

Structures

Toxin

• PDB: 4C26
• PDB: 6G26

Antitoxin

• AlphaFold DB: Q2T3Q7