Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT_toxin-BrnA |
| Location | 3225341..3225878 | Replicon | chromosome |
| Accession | NZ_CP008784 | ||
| Organism | Burkholderia pseudomallei MSHR5855 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | Q2T6G2 |
| Locus tag | DP63_RS27605 | Protein ID | WP_004549731.1 |
| Coordinates | 3225609..3225878 (-) | Length | 90 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | Q63NA3 |
| Locus tag | DP63_RS27600 | Protein ID | WP_004525420.1 |
| Coordinates | 3225341..3225625 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DP63_RS40150 | 3220628..3220813 | - | 186 | WP_024429283.1 | hypothetical protein | - |
| DP63_RS27575 | 3220810..3222525 | - | 1716 | WP_024429282.1 | terminase large subunit | - |
| DP63_RS27580 | 3222529..3223005 | - | 477 | WP_004549538.1 | phage terminase small subunit P27 family | - |
| DP63_RS38300 | 3223143..3223499 | - | 357 | WP_024429281.1 | HNH endonuclease | - |
| DP63_RS27590 | 3223588..3224166 | - | 579 | WP_038738294.1 | hypothetical protein | - |
| DP63_RS27595 | 3224168..3225079 | - | 912 | WP_143281104.1 | hypothetical protein | - |
| DP63_RS27600 | 3225341..3225625 | - | 285 | WP_004525420.1 | BrnA antitoxin family protein | Antitoxin |
| DP63_RS27605 | 3225609..3225878 | - | 270 | WP_004549731.1 | BrnT family toxin | Toxin |
| DP63_RS27610 | 3225927..3226370 | - | 444 | WP_004548453.1 | hypothetical protein | - |
| DP63_RS27615 | 3226367..3226627 | - | 261 | WP_024429361.1 | hypothetical protein | - |
| DP63_RS38305 | 3226603..3226935 | - | 333 | WP_038725178.1 | hypothetical protein | - |
| DP63_RS27620 | 3226965..3227363 | - | 399 | WP_038738296.1 | DUF1364 family protein | - |
| DP63_RS38310 | 3227378..3227725 | - | 348 | WP_043289604.1 | DUF2591 family protein | - |
| DP63_RS27625 | 3227722..3228051 | - | 330 | WP_024429362.1 | winged helix-turn-helix domain-containing protein | - |
| DP63_RS27630 | 3228076..3228612 | - | 537 | WP_024429363.1 | DUF1064 domain-containing protein | - |
| DP63_RS27635 | 3228609..3229601 | - | 993 | WP_038738301.1 | hypothetical protein | - |
| DP63_RS27640 | 3229755..3230015 | - | 261 | WP_004526650.1 | helix-turn-helix domain-containing protein | - |
| DP63_RS27645 | 3230012..3230833 | - | 822 | WP_015967391.1 | chromosome partitioning protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3180461..3249096 | 68635 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 90 a.a. Molecular weight: 10157.46 Da Isoelectric Point: 5.6831
>T47438 WP_004549731.1 NZ_CP008784:c3225878-3225609 [Burkholderia pseudomallei MSHR5855]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA
Download Length: 270 bp
>T47438 NZ_CP008784:c3225878-3225609 [Burkholderia pseudomallei MSHR5855]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10697.10 Da Isoelectric Point: 5.8930
>AT47438 WP_004525420.1 NZ_CP008784:c3225625-3225341 [Burkholderia pseudomallei MSHR5855]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA
Download Length: 285 bp
>AT47438 NZ_CP008784:c3225625-3225341 [Burkholderia pseudomallei MSHR5855]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q2T6G2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0F6L9S8 |