Detailed information of TA system TA047432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2110601..2111042
• Replicon: chromosome
• Accession: NZ_CP008782
• Organism: Burkholderia pseudomallei strain Mahidol-1106a

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: DP58_RS30270
• Protein ID: WP_009939402.1
• Coordinates: 2110869..2111042 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DP58_RS26150
• Protein ID: WP_004525420.1
• Coordinates: 2110601..2110885 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP58_RS26125	2105690..2106952	-	1263	WP_004537187.1	c-type cytochrome	-
DP58_RS26130	2107111..2108571	-	1461	WP_004536920.1	metallophosphoesterase	-
DP58_RS26135	2108837..2108953	+	117	Protein_1674	IS3 family transposase	-
DP58_RS36405	2109180..2109608	-	429	Protein_1675	IS110 family transposase	-
DP58_RS26140	2109571..2109954	-	384	Protein_1676	DDE-type integrase/transposase/recombinase	-
DP58_RS26150	2110601..2110885	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DP58_RS30270	2110869..2111042	-	174	WP_009939402.1	BrnT family toxin	Toxin
DP58_RS26155	2111710..2111985	+	276	WP_004537565.1	bacteriophage protein Gp49	-
DP58_RS39825	2112265..2112441	+	177	WP_004552958.1	hypothetical protein	-
DP58_RS26170	2112470..2112817	+	348	WP_009946438.1	hypothetical protein	-
DP58_RS39440	2112865..2113083	+	219	WP_004549687.1	hypothetical protein	-
DP58_RS26180	2113258..2113848	-	591	WP_009946442.1	hypothetical protein	-
DP58_RS26185	2114098..2114247	+	150	WP_004537646.1	bacteriophage protein Gp44	-
DP58_RS39830	2114244..2115017	+	774	Protein_1685	prohibitin family protein	-
DP58_RS39835	2115015..2115443	+	429	Protein_1686	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2107111..2117754	10643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T47432 WP_009939402.1 NZ_CP008782:c2111042-2110869 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T47432 NZ_CP008782:c2111042-2110869 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47432 WP_004525420.1 NZ_CP008782:c2110885-2110601 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47432 NZ_CP008782:c2110885-2110601 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8