Detailed information of TA system TA047429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2694503..2695158
• Replicon: chromosome
• Accession: NZ_CP008780
• Organism: Burkholderia pseudomallei strain MSHR1655

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DP51_RS24260
• Protein ID: WP_004521942.1
• Coordinates: 2694862..2695158 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: DP51_RS24255
• Protein ID: WP_004202809.1
• Coordinates: 2694503..2694859 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP51_RS24225	2690629..2691387	+	759	WP_004521940.1	cytochrome c1	-
DP51_RS24230	2691480..2692091	+	612	WP_004185176.1	glutathione S-transferase N-terminal domain-containing protein	-
DP51_RS24235	2692161..2692688	+	528	WP_004521941.1	ClpXP protease specificity-enhancing factor	-
DP51_RS24245	2692910..2693407	+	498	WP_004199888.1	terminase	-
DP51_RS24250	2693404..2694459	+	1056	WP_004557286.1	phage portal protein	-
DP51_RS24255	2694503..2694859	-	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
DP51_RS24260	2694862..2695158	-	297	WP_004521942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DP51_RS36610	2695826..2697082	+	1257	WP_004552667.1	AAA family ATPase	-
DP51_RS24265	2697127..2697702	+	576	WP_198014201.1	DUF4276 family protein	-
DP51_RS24270	2698474..2698800	+	327	WP_004521944.1	baseplate assembly protein	-
DP51_RS36625	2698793..2699204	+	412	Protein_2351	phage tail protein I	-
DP51_RS24275	2699102..2699704	+	603	WP_076857191.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2693093..2701160	8067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10902.68 Da
• Isoelectric Point: 9.9369

>T47429 WP_004521942.1 NZ_CP008780:c2695158-2694862 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIADELED

Nucleotide

• Length: 297 bp

>T47429 NZ_CP008780:c2695158-2694862 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGACGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT47429 WP_004202809.1 NZ_CP008780:c2694859-2694503 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT47429 NZ_CP008780:c2694859-2694503 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3