Detailed information of TA system TA047428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1721334..1721871
• Replicon: chromosome
• Accession: NZ_CP008779
• Organism: Burkholderia pseudomallei strain MSHR1655

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A2K9CXB8
• Locus tag: DP51_RS06820
• Protein ID: WP_004557717.1
• Coordinates: 1721602..1721871 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DP51_RS06815
• Protein ID: WP_004525420.1
• Coordinates: 1721334..1721618 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP51_RS06790	1716442..1717704	-	1263	WP_004530738.1	c-type cytochrome	-
DP51_RS06795	1717863..1719323	-	1461	WP_004525423.1	metallophosphoesterase	-
DP51_RS06800	1719570..1719686	+	117	Protein_1350	IS3 family transposase	-
DP51_RS32470	1719913..1720341	-	429	Protein_1351	IS110 family transposase	-
DP51_RS06805	1720304..1720687	-	384	Protein_1352	DDE-type integrase/transposase/recombinase	-
DP51_RS06815	1721334..1721618	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DP51_RS06820	1721602..1721871	-	270	WP_004557717.1	BrnT family toxin	Toxin
DP51_RS06825	1722424..1722699	+	276	WP_004525419.1	hypothetical protein	-
DP51_RS06830	1722709..1722840	+	132	WP_009923452.1	bacteriophage protein Gp48	-
DP51_RS06840	1722976..1723197	+	222	WP_004525418.1	hypothetical protein	-
DP51_RS39285	1723245..1723463	+	219	WP_004552392.1	hypothetical protein	-
DP51_RS06850	1723694..1724275	-	582	WP_004542548.1	hypothetical protein	-
DP51_RS06855	1724531..1724680	+	150	WP_004542626.1	bacteriophage protein Gp44	-
DP51_RS39835	1724677..1725450	+	774	Protein_1361	prohibitin family protein	-
DP51_RS39840	1725448..1725876	+	429	Protein_1362	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1717863..1728187	10324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10183.54 Da
• Isoelectric Point: 5.6831

>T47428 WP_004557717.1 NZ_CP008779:c1721871-1721602 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDLMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T47428 NZ_CP008779:c1721871-1721602 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGATGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTTGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47428 WP_004525420.1 NZ_CP008779:c1721618-1721334 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47428 NZ_CP008779:c1721618-1721334 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: A0A2K9CXB8

Antitoxin

• AlphaFold DB: A0A0F6L9S8