Detailed information of TA system TA047425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 3147651..3148188
• Replicon: chromosome
• Accession: NZ_CP008778
• Organism: Burkholderia pseudomallei 576

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: DP50_RS30795
• Protein ID: WP_004549731.1
• Coordinates: 3147919..3148188 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DP50_RS30790
• Protein ID: WP_004525420.1
• Coordinates: 3147651..3147935 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP50_RS30765	3142786..3144021	-	1236	WP_100086160.1	c-type cytochrome	-
DP50_RS30770	3144180..3145640	-	1461	WP_004545516.1	metallophosphoesterase	-
DP50_RS30775	3145887..3146003	+	117	Protein_2530	IS3 family transposase	-
DP50_RS38925	3146230..3146658	-	429	Protein_2531	IS110 family transposase	-
DP50_RS30780	3146621..3147004	-	384	Protein_2532	DDE-type integrase/transposase/recombinase	-
DP50_RS30790	3147651..3147935	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DP50_RS30795	3147919..3148188	-	270	WP_004549731.1	BrnT family toxin	Toxin
DP50_RS30805	3148758..3149033	+	276	WP_004537565.1	bacteriophage protein Gp49	-
DP50_RS30810	3149043..3149174	+	132	WP_009923452.1	bacteriophage protein Gp48	-
DP50_RS30820	3149312..3149554	+	243	WP_004545584.1	hypothetical protein	-
DP50_RS41335	3149551..3149709	+	159	WP_004545508.1	hypothetical protein	-
DP50_RS41020	3149757..3149975	+	219	WP_004552392.1	hypothetical protein	-
DP50_RS38940	3150161..3150814	-	654	WP_004545536.1	hypothetical protein	-
DP50_RS30840	3151100..3152320	+	1221	WP_041286571.1	ISL3-like element ISBma1 family transposase	-
DP50_RS30845	3152378..3152527	+	150	WP_004537646.1	bacteriophage protein Gp44	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3140530..3174560	34030
	-	inside	IScluster/Tn	-	-	3140530..3165250	24720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T47425 WP_004549731.1 NZ_CP008778:c3148188-3147919 [Burkholderia pseudomallei 576]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T47425 NZ_CP008778:c3148188-3147919 [Burkholderia pseudomallei 576]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGATGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47425 WP_004525420.1 NZ_CP008778:c3147935-3147651 [Burkholderia pseudomallei 576]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47425 NZ_CP008778:c3147935-3147651 [Burkholderia pseudomallei 576]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8