Detailed information of TA system TA047419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1986689..1987293
• Replicon: chromosome
• Accession: NZ_CP008762
• Organism: Paraburkholderia xenovorans LB400

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DR64_RS36980
• Protein ID: WP_193352970.1
• Coordinates: 1986988..1987293 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q13LE6
• Locus tag: DR64_RS36975
• Protein ID: WP_011492395.1
• Coordinates: 1986689..1986991 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR64_RS36950	1982022..1983350	+	1329	WP_040124527.1	Nramp family divalent metal transporter	-
DR64_RS36955	1983503..1984072	-	570	WP_011492398.1	flagellar transcriptional regulator FlhC	-
DR64_RS36960	1984087..1984407	-	321	WP_007177882.1	flagellar transcriptional regulator FlhD	-
DR64_RS36965	1985043..1985576	-	534	WP_011492397.1	GNAT family N-acetyltransferase	-
DR64_RS36970	1985651..1986553	-	903	WP_081435596.1	helix-turn-helix transcriptional regulator	-
DR64_RS36975	1986689..1986991	-	303	WP_011492395.1	putative addiction module antidote protein	Antitoxin
DR64_RS36980	1986988..1987293	-	306	WP_193352970.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DR64_RS36985	1987718..1988086	+	369	WP_011492393.1	nuclear transport factor 2 family protein	-
DR64_RS36990	1988112..1988345	+	234	Protein_1733	hypothetical protein	-
DR64_RS36995	1988628..1989092	+	465	WP_011492391.1	transporter RbsD/FucU	-
DR64_RS37000	1989251..1989658	+	408	WP_011492390.1	VOC family protein	-
DR64_RS37005	1989870..1990328	+	459	WP_011492389.1	DoxX family protein	-
DR64_RS37010	1990393..1990665	+	273	WP_011492388.1	hypothetical protein	-
DR64_RS37020	1991537..1992052	+	516	WP_049782302.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11763.46 Da
• Isoelectric Point: 8.8770

>T47419 WP_193352970.1 NZ_CP008762:c1987293-1986988 [Paraburkholderia xenovorans LB400]
VYTVNRTEEFDAWLARLSDQRARAKILVRIRRAERGHFGDMKLLEDGVSEMRIDYGPGYRVYFAREERVVYLLLCGGDKS
TQPADIKHAKTMWAAIREELS

Nucleotide

• Length: 306 bp

>T47419 NZ_CP008762:c1987293-1986988 [Paraburkholderia xenovorans LB400]
GTGTACACGGTCAACCGCACCGAGGAGTTCGACGCCTGGCTCGCGCGGCTCTCCGATCAAAGAGCGAGGGCGAAAATACT
CGTGCGAATCCGGCGTGCGGAACGCGGGCATTTCGGCGACATGAAGTTGCTGGAAGACGGTGTGTCAGAGATGCGCATCG
ACTACGGCCCGGGTTATCGGGTCTACTTCGCGCGTGAAGAACGCGTGGTCTATCTGCTGCTCTGCGGTGGCGACAAGTCC
ACCCAGCCAGCCGATATCAAGCATGCCAAAACAATGTGGGCAGCAATCAGAGAGGAACTGTCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10545.22 Da
• Isoelectric Point: 5.8505

>AT47419 WP_011492395.1 NZ_CP008762:c1986991-1986689 [Paraburkholderia xenovorans LB400]
MSKIKTARFDASHYLDSEEMIAEYLNAALEEGDADLLLAAIADIAKARGIAKIAADAGLGRESLYKTLAPGSKPRMDTVF
KLLRALGVKLNAVPEGVAAA

Nucleotide

• Length: 303 bp

>AT47419 NZ_CP008762:c1986991-1986689 [Paraburkholderia xenovorans LB400]
ATGAGCAAGATCAAAACCGCACGGTTCGACGCGTCACACTACCTCGATAGCGAGGAAATGATCGCCGAGTATCTCAACGC
CGCGCTGGAAGAAGGCGACGCCGACCTCCTGCTCGCCGCAATCGCCGACATCGCCAAAGCGCGCGGCATCGCCAAGATCG
CGGCAGATGCCGGACTTGGCCGCGAAAGCCTCTACAAAACGCTCGCGCCCGGTTCCAAACCACGCATGGATACGGTGTTC
AAGCTGCTGCGCGCGCTCGGCGTCAAACTCAACGCGGTGCCGGAAGGCGTGGCGGCGGCCTGA

Structures

Antitoxin

• AlphaFold DB: Q13LE6