Detailed information of TA system TA047415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2543874..2544463
• Replicon: chromosome
• Accession: NZ_CP008760
• Organism: Paraburkholderia xenovorans LB400

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I2IER1
• Locus tag: DR64_RS11340
• Protein ID: WP_007179958.1
• Coordinates: 2544281..2544463 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q13SZ3
• Locus tag: DR64_RS11335
• Protein ID: WP_011490213.1
• Coordinates: 2543874..2544284 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR64_RS11320	2540207..2542180	+	1974	WP_011490215.1	flagellar biosynthesis protein FlhF	-
DR64_RS11325	2542173..2543042	+	870	WP_038457172.1	MinD/ParA family protein	-
DR64_RS11330	2543071..2543805	+	735	WP_007179960.1	RNA polymerase sigma factor FliA	-
DR64_RS11335	2543874..2544284	-	411	WP_011490213.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DR64_RS11340	2544281..2544463	-	183	WP_007179958.1	type II toxin-antitoxin system HicA family toxin	Toxin
DR64_RS11345	2544772..2545218	-	447	WP_011490212.1	flagellar protein FlgN	-
DR64_RS11350	2545313..2545666	-	354	WP_011490211.1	flagellar biosynthesis anti-sigma factor FlgM	-
DR64_RS11355	2545880..2547388	-	1509	WP_011490210.1	flagellar basal body P-ring formation protein FlgA	-
DR64_RS11360	2547606..2548100	+	495	WP_011490209.1	flagellar basal body rod protein FlgB	-
DR64_RS11365	2548249..2548674	+	426	WP_007179953.1	flagellar basal body rod protein FlgC	-
DR64_RS11370	2548685..2549407	+	723	WP_011490208.1	flagellar hook assembly protein FlgD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (6-129)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6571.77 Da
• Isoelectric Point: 12.1412

>T47415 WP_007179958.1 NZ_CP008760:c2544463-2544281 [Paraburkholderia xenovorans LB400]
MKSAEVMKLIQAAGWRLIRISGSHHHFRHAARAGLVTIPHPNKDLPPGTLNSILKQAGLK

Nucleotide

• Length: 183 bp

>T47415 NZ_CP008760:c2544463-2544281 [Paraburkholderia xenovorans LB400]
ATGAAAAGCGCCGAGGTTATGAAACTCATTCAAGCGGCTGGCTGGCGGCTGATCCGCATATCGGGAAGCCACCATCATTT
CCGTCACGCGGCAAGAGCCGGTCTCGTGACCATCCCGCATCCGAACAAAGACCTTCCTCCCGGCACGTTGAACAGCATCT
TGAAACAGGCGGGACTGAAATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14708.72 Da
• Isoelectric Point: 4.5106

>AT47415 WP_011490213.1 NZ_CP008760:c2544284-2543874 [Paraburkholderia xenovorans LB400]
MKNLTFPIAIEPGNADHAFGVIVPDIPGCYAAGDTLEEAYANAKEAIEAHLDLLLDEGLPVPARLSLEEHRRNPDYADFT
WGLVATRNIPALKKAVRINISLPEALVQEIDTYAQARGMSRSAFLALAAEHEMAGA

Nucleotide

• Length: 411 bp

>AT47415 NZ_CP008760:c2544284-2543874 [Paraburkholderia xenovorans LB400]
ATGAAAAACCTGACTTTTCCGATCGCGATTGAACCGGGCAATGCGGATCACGCGTTCGGCGTGATCGTGCCCGACATCCC
GGGTTGTTACGCAGCAGGCGATACGCTGGAAGAGGCCTACGCAAACGCGAAAGAAGCCATTGAAGCGCATCTTGATCTGC
TGCTCGACGAAGGCTTGCCGGTCCCTGCGCGACTCTCGCTGGAGGAGCATCGGCGCAATCCCGACTATGCGGATTTCACG
TGGGGGCTTGTCGCCACCCGGAATATTCCCGCCTTGAAGAAGGCGGTGCGTATCAATATCTCGTTGCCCGAAGCGTTGGT
TCAGGAAATCGACACTTACGCCCAGGCGCGCGGCATGTCGCGCTCGGCGTTTCTGGCGCTGGCCGCGGAACATGAAATGG
CCGGTGCATGA

Structures

Toxin

• AlphaFold DB: I2IER1

Antitoxin

• AlphaFold DB: Q13SZ3