Detailed information of TA system TA047412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 368107..368548
• Replicon: chromosome
• Accession: NZ_CP008759
• Organism: Burkholderia pseudomallei strain 1106a

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: DP48_RS30195
• Protein ID: WP_009939402.1
• Coordinates: 368375..368548 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DP48_RS19175
• Protein ID: WP_004525420.1
• Coordinates: 368107..368391 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP48_RS19150	363196..364458	-	1263	WP_004537187.1	c-type cytochrome	-
DP48_RS19155	364617..366077	-	1461	WP_004536920.1	metallophosphoesterase	-
DP48_RS19160	366343..366459	+	117	Protein_286	IS3 family transposase	-
DP48_RS34155	366686..367114	-	429	Protein_287	IS110 family transposase	-
DP48_RS19165	367077..367460	-	384	Protein_288	DDE-type integrase/transposase/recombinase	-
DP48_RS19175	368107..368391	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DP48_RS30195	368375..368548	-	174	WP_009939402.1	BrnT family toxin	Toxin
DP48_RS19180	369216..369491	+	276	WP_004537565.1	bacteriophage protein Gp49	-
DP48_RS39650	369771..369947	+	177	WP_004552958.1	hypothetical protein	-
DP48_RS19195	369976..370323	+	348	WP_009946438.1	hypothetical protein	-
DP48_RS39210	370371..370589	+	219	WP_004549687.1	hypothetical protein	-
DP48_RS19205	370764..371354	-	591	WP_009946442.1	hypothetical protein	-
DP48_RS39810	371604..371753	+	150	WP_004537646.1	bacteriophage protein Gp44	-
DP48_RS39660	371750..372523	+	774	Protein_297	prohibitin family protein	-
DP48_RS39815	372521..372949	+	429	Protein_298	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	360975..375260	14285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T47412 WP_009939402.1 NZ_CP008759:c368548-368375 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T47412 NZ_CP008759:c368548-368375 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47412 WP_004525420.1 NZ_CP008759:c368391-368107 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47412 NZ_CP008759:c368391-368107 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8