Detailed information of TA system TA047411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1516066..1516625
• Replicon: chromosome
• Accession: NZ_CP008757
• Organism: Brucella suis strain BSP

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: DO76_RS13885
• Protein ID: WP_004688321.1
• Coordinates: 1516380..1516625 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: DO76_RS13880
• Protein ID: WP_002964097.1
• Coordinates: 1516066..1516380 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DO76_RS13830	1511756..1511959	-	204	WP_004690812.1	hypothetical protein	-
DO76_RS13835	1511962..1512177	-	216	WP_002971459.1	hypothetical protein	-
DO76_RS13840	1512174..1512377	-	204	WP_002964088.1	hypothetical protein	-
DO76_RS13845	1512427..1513146	+	720	WP_006072696.1	hypothetical protein	-
DO76_RS13850	1513143..1513373	+	231	WP_002964091.1	hypothetical protein	-
DO76_RS13855	1513370..1513645	+	276	WP_002964092.1	hypothetical protein	-
DO76_RS13860	1513668..1514255	+	588	WP_004683738.1	hypothetical protein	-
DO76_RS13865	1514490..1515200	+	711	WP_004683739.1	porin family protein	-
DO76_RS13870	1515548..1515718	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
DO76_RS13880	1516066..1516380	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
DO76_RS13885	1516380..1516625	-	246	WP_004688321.1	BrnT family toxin	Toxin
DO76_RS13890	1516758..1517396	+	639	Protein_1429	tRNA-guanine transglycosylase	-
DO76_RS17455	1517466..1518223	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
DO76_RS13905	1518224..1518997	+	774	WP_006132649.1	hypothetical protein	-
DO76_RS13910	1519132..1520778	+	1647	Protein_1432	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	1509984..1524094	14110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T47411 WP_004688321.1 NZ_CP008757:c1516625-1516380 [Brucella suis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T47411 NZ_CP008757:c1516625-1516380 [Brucella suis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT47411 WP_002964097.1 NZ_CP008757:c1516380-1516066 [Brucella suis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT47411 NZ_CP008757:c1516380-1516066 [Brucella suis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA