Detailed information of TA system TA047409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2348620..2349109
• Replicon: chromosome
• Accession: NZ_CP008754
• Organism: Burkholderia pseudomallei strain 9

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q63NA2
• Locus tag: DP46_RS11910
• Protein ID: WP_004547086.1
• Coordinates: 2348620..2348841 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DP46_RS11915
• Protein ID: WP_004525420.1
• Coordinates: 2348825..2349109 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP46_RS11870	2344267..2345466	-	1200	WP_004537208.1	DDE-type integrase/transposase/recombinase	-
DP46_RS11875	2345463..2345612	-	150	WP_004537646.1	bacteriophage protein Gp44	-
DP46_RS11880	2345754..2346452	+	699	WP_076805202.1	hypothetical protein	-
DP46_RS11885	2346642..2346896	+	255	WP_004552959.1	hypothetical protein	-
DP46_RS11890	2346893..2347240	-	348	WP_009946438.1	hypothetical protein	-
DP46_RS11895	2347475..2347582	-	108	WP_009946436.1	hypothetical protein	-
DP46_RS11900	2347584..2347715	-	132	WP_015967385.1	hypothetical protein	-
DP46_RS11905	2347725..2348000	-	276	WP_004537565.1	bacteriophage protein Gp49	-
DP46_RS11910	2348620..2348841	+	222	WP_004547086.1	BrnT family toxin	Toxin
DP46_RS11915	2348825..2349109	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DP46_RS11920	2349419..2349616	-	198	WP_076823250.1	hypothetical protein	-
DP46_RS11925	2349527..2349766	+	240	WP_004536695.1	HNH endonuclease	-
DP46_RS11930	2349756..2350139	+	384	Protein_2376	DDE-type integrase/transposase/recombinase	-
DP46_RS11935	2350102..2350530	+	429	Protein_2377	IS110 family transposase	-
DP46_RS11940	2350757..2350873	-	117	Protein_2378	IS3 family transposase	-
DP46_RS11945	2351139..2352599	+	1461	WP_004547073.1	metallophosphoesterase	-
DP46_RS11950	2352650..2354020	+	1371	WP_011857641.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ddhA	2335613..2413830	78217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (14-65)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8634.77 Da
• Isoelectric Point: 8.4920

>T47409 WP_004547086.1 NZ_CP008754:2348620-2348841 [Burkholderia pseudomallei]
MECRWRLQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 222 bp

>T47409 NZ_CP008754:2348620-2348841 [Burkholderia pseudomallei]
ATGGAGTGTCGTTGGCGCTTGCAGCGCAACTCGACTGGTCAGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAG
TGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGC
ACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47409 WP_004525420.1 NZ_CP008754:2348825-2349109 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47409 NZ_CP008754:2348825-2349109 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q63NA2

Antitoxin

• AlphaFold DB: A0A0F6L9S8