Detailed information of TA system TA047407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 961181..961740
• Replicon: chromosome
• Accession: NZ_CP008750
• Organism: Brucella melitensis strain 20236 isolate Bme20236

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: BME20236_RS04675
• Protein ID: WP_002966807.1
• Coordinates: 961495..961740 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: BME20236_RS04670
• Protein ID: WP_004683740.1
• Coordinates: 961181..961495 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME20236_RS04625	956886..957089	-	204	WP_004683737.1	hypothetical protein	-
BME20236_RS04630	957092..957307	-	216	WP_005968947.1	hypothetical protein	-
BME20236_RS04635	957304..957507	-	204	WP_002964088.1	hypothetical protein	-
BME20236_RS04640	957557..958261	+	705	WP_005968944.1	hypothetical protein	-
BME20236_RS04645	958258..958488	+	231	WP_002964091.1	hypothetical protein	-
BME20236_RS04650	958485..958760	+	276	WP_002964092.1	hypothetical protein	-
BME20236_RS04655	958783..959259	+	477	WP_002966806.1	hypothetical protein	-
BME20236_RS04660	959605..960315	+	711	WP_004683739.1	porin family protein	-
BME20236_RS04665	960663..960833	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BME20236_RS04670	961181..961495	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
BME20236_RS04675	961495..961740	-	246	WP_002966807.1	BrnT family toxin	Toxin
BME20236_RS04680	961873..962511	+	639	Protein_922	tRNA-guanine transglycosylase	-
BME20236_RS17495	962581..963339	+	759	Protein_923	IS5 family transposase	-
BME20236_RS04690	963340..964032	+	693	WP_006135935.1	hypothetical protein	-
BME20236_RS04695	964444..965895	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	955114..969211	14097
	-	flank	IS/Tn	-	-	962581..962949	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T47407 WP_002966807.1 NZ_CP008750:c961740-961495 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T47407 NZ_CP008750:c961740-961495 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT47407 WP_004683740.1 NZ_CP008750:c961495-961181 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT47407 NZ_CP008750:c961495-961181 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1