Detailed information of TA system TA047400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3780053..3780582
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: GS11_RS17705
• Protein ID: WP_003417760.1
• Coordinates: 3780325..3780582 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: GS11_RS17700
• Protein ID: WP_003417757.1
• Coordinates: 3780053..3780328 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS17665	3775084..3776382	-	1299	WP_011799343.1	PPE family protein	-
GS11_RS22630	3776851..3778288	-	1438	Protein_3454	FAD-binding oxidoreductase	-
GS11_RS17685	3778391..3778780	+	390	WP_010950876.1	DUF732 domain-containing protein	-
GS11_RS17690	3778794..3779087	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
GS11_RS17695	3779084..3779929	-	846	WP_010950877.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
GS11_RS17700	3780053..3780328	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
GS11_RS17705	3780325..3780582	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
GS11_RS17710	3780624..3781814	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
GS11_RS17715	3781931..3782299	+	369	WP_003417765.1	FHA domain-containing protein	-
GS11_RS17720	3782296..3782847	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
GS11_RS17725	3782854..3783435	-	582	WP_003417769.1	ATP/GTP-binding protein	-
GS11_RS17730	3783416..3783784	-	369	WP_003417772.1	DUF742 domain-containing protein	-
GS11_RS17735	3783762..3784154	-	393	WP_011799344.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T47400 WP_003417760.1 NZ_CP008744:3780325-3780582 [Mycobacterium tuberculosis variant bovis BCG]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T47400 NZ_CP008744:3780325-3780582 [Mycobacterium tuberculosis variant bovis BCG]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT47400 WP_003417757.1 NZ_CP008744:3780053-3780328 [Mycobacterium tuberculosis variant bovis BCG]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT47400 NZ_CP008744:3780053-3780328 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94