Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3717922..3718596 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag GS11_RS17495 Protein ID WP_003417282.1
Coordinates 3717922..3718350 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag GS11_RS17500 Protein ID WP_003417286.1
Coordinates 3718354..3718596 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS17470 3713744..3714145 - 402 WP_003417264.1 cytidine deaminase -
GS11_RS17475 3714286..3714720 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
GS11_RS17480 3714717..3715151 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
GS11_RS17485 3715280..3717052 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
GS11_RS17490 3717052..3717843 + 792 WP_010950870.1 succinate dehydrogenase iron-sulfur subunit -
GS11_RS17495 3717922..3718350 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
GS11_RS17500 3718354..3718596 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
GS11_RS17505 3718718..3719332 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
GS11_RS17510 3719329..3719994 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
GS11_RS17515 3719995..3720528 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
GS11_RS17520 3720525..3720899 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
GS11_RS17525 3720996..3722060 - 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T47399 WP_003417282.1 NZ_CP008744:c3718350-3717922 [Mycobacterium tuberculosis variant bovis BCG]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T47399 NZ_CP008744:c3718350-3717922 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT47399 WP_003417286.1 NZ_CP008744:c3718596-3718354 [Mycobacterium tuberculosis variant bovis BCG]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT47399 NZ_CP008744:c3718596-3718354 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References