Detailed information of TA system TA047396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3116553..3117240
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7U4FBY8
• Locus tag: GS11_RS14785
• Protein ID: WP_011799298.1
• Coordinates: 3116797..3117240 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: GS11_RS14780
• Protein ID: WP_003414620.1
• Coordinates: 3116553..3116810 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS14760	3111873..3112727	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
GS11_RS14765	3112783..3113946	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
GS11_RS14770	3113963..3115177	-	1215	WP_011799297.1	zinc metalloprotease Rip	-
GS11_RS14775	3115185..3116426	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
GS11_RS14780	3116553..3116810	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
GS11_RS14785	3116797..3117240	+	444	WP_011799298.1	type II toxin-antitoxin system VapC family toxin	Toxin
GS11_RS14790	3117320..3117982	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
GS11_RS14795	3118078..3118269	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GS11_RS14800	3118601..3120349	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
GS11_RS14805	3120445..3121026	+	582	WP_003414644.1	fasciclin domain-containing protein	-
GS11_RS14810	3121126..3121392	+	267	WP_031657293.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16625.74 Da
• Isoelectric Point: 6.4890

>T47396 WP_011799298.1 NZ_CP008744:3116797-3117240 [Mycobacterium tuberculosis variant bovis BCG]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDTLLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T47396 NZ_CP008744:3116797-3117240 [Mycobacterium tuberculosis variant bovis BCG]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACACCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT47396 WP_003414620.1 NZ_CP008744:3116553-3116810 [Mycobacterium tuberculosis variant bovis BCG]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT47396 NZ_CP008744:3116553-3116810 [Mycobacterium tuberculosis variant bovis BCG]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBY8

Antitoxin

• AlphaFold DB: G0TFH0