Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3070089..3070693 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag GS11_RS14550 Protein ID WP_003414492.1
Coordinates 3070089..3070481 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag GS11_RS14555 Protein ID WP_003414495.1
Coordinates 3070478..3070693 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS14520 3065239..3066027 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
GS11_RS14525 3066361..3066906 - 546 WP_003904931.1 DUF1802 family protein -
GS11_RS14530 3067178..3068062 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
GS11_RS14535 3068065..3068952 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
GS11_RS14540 3069257..3069802 - 546 WP_003899500.1 DUF1802 family protein -
GS11_RS14545 3069799..3070068 - 270 WP_003414489.1 DUF2277 family protein -
GS11_RS14550 3070089..3070481 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
GS11_RS14555 3070478..3070693 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GS11_RS14560 3070740..3071489 + 750 WP_003414497.1 enoyl-CoA hydratase -
GS11_RS14565 3071568..3072650 - 1083 WP_011799293.1 ABC transporter ATP-binding protein -
GS11_RS21470 3072643..3073953 - 1311 Protein_2839 ABC transporter substrate-binding protein -
GS11_RS14580 3073956..3074783 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
GS11_RS14585 3074780..3075691 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T47394 WP_003414492.1 NZ_CP008744:c3070481-3070089 [Mycobacterium tuberculosis variant bovis BCG]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T47394 NZ_CP008744:c3070481-3070089 [Mycobacterium tuberculosis variant bovis BCG]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT47394 WP_003414495.1 NZ_CP008744:c3070693-3070478 [Mycobacterium tuberculosis variant bovis BCG]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT47394 NZ_CP008744:c3070693-3070478 [Mycobacterium tuberculosis variant bovis BCG]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References