Detailed information of TA system TA047392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3043096..3043666
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: GS11_RS14400
• Protein ID: WP_003414166.1
• Coordinates: 3043096..3043452 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: GS11_RS14405
• Protein ID: WP_003901465.1
• Coordinates: 3043436..3043666 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS14380	3038548..3040236	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
GS11_RS14385	3040240..3040566	-	327	WP_003414157.1	hypothetical protein	-
GS11_RS14390	3040739..3041326	+	588	WP_003914429.1	DUF3558 family protein	-
GS11_RS14395	3041345..3042994	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
GS11_RS14400	3043096..3043452	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
GS11_RS14405	3043436..3043666	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
GS11_RS14410	3043709..3044752	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
GS11_RS22085	3044943..3045218	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
GS11_RS22090	3045394..3045648	-	255	WP_003917684.1	hypothetical protein	-
GS11_RS14425	3045796..3046200	+	405	WP_003414181.1	hypothetical protein	-
GS11_RS14430	3046197..3046388	+	192	WP_003414184.1	hypothetical protein	-
GS11_RS14435	3046452..3047741	+	1290	Protein_2813	transposase family protein	-
GS11_RS14440	3047975..3048232	+	258	WP_003899489.1	hypothetical protein	-
GS11_RS14445	3048337..3048648	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T47392 WP_003414166.1 NZ_CP008744:c3043452-3043096 [Mycobacterium tuberculosis variant bovis BCG]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T47392 NZ_CP008744:c3043452-3043096 [Mycobacterium tuberculosis variant bovis BCG]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT47392 WP_003901465.1 NZ_CP008744:c3043666-3043436 [Mycobacterium tuberculosis variant bovis BCG]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT47392 NZ_CP008744:c3043666-3043436 [Mycobacterium tuberculosis variant bovis BCG]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3