Detailed information of TA system TA047390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3003100..3003779
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: GS11_RS14170
• Protein ID: WP_003414059.1
• Coordinates: 3003100..3003516 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: GS11_RS14175
• Protein ID: WP_003414061.1
• Coordinates: 3003513..3003779 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS14150	2999152..3000054	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GS11_RS14155	3000123..3000875	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
GS11_RS14160	3001119..3001394	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
GS11_RS14165	3001391..3003013	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
GS11_RS14170	3003100..3003516	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
GS11_RS14175	3003513..3003779	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GS11_RS14180	3003805..3004200	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
GS11_RS14185	3004197..3004466	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
GS11_RS14190	3004476..3005570	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
GS11_RS14195	3005567..3005986	-	420	Protein_2766	winged helix-turn-helix domain-containing protein	-
GS11_RS14200	3006060..3006539	-	480	WP_003414073.1	dihydrofolate reductase	-
GS11_RS14205	3006610..3007410	-	801	WP_003911953.1	thymidylate synthase	-
GS11_RS14210	3007566..3008303	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T47390 WP_003414059.1 NZ_CP008744:c3003516-3003100 [Mycobacterium tuberculosis variant bovis BCG]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T47390 NZ_CP008744:c3003516-3003100 [Mycobacterium tuberculosis variant bovis BCG]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT47390 WP_003414061.1 NZ_CP008744:c3003779-3003513 [Mycobacterium tuberculosis variant bovis BCG]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT47390 NZ_CP008744:c3003779-3003513 [Mycobacterium tuberculosis variant bovis BCG]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64