47388

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2813659..2814296 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag GS11_RS13180 Protein ID WP_003413180.1
Coordinates 2813659..2814054 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag GS11_RS13185 Protein ID WP_003413183.1
Coordinates 2814051..2814296 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS13140 2809063..2810274 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
GS11_RS13145 2810401..2811059 + 659 Protein_2555 LppA family lipoprotein -
GS11_RS13150 2811056..2811718 + 663 WP_048657336.1 lipoprotein LppA -
GS11_RS13155 2811715..2811993 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
GS11_RS13160 2812086..2812499 + 414 WP_003413164.1 PIN domain nuclease -
GS11_RS13165 2812538..2812795 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
GS11_RS13170 2812792..2813169 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
GS11_RS13175 2813185..2813559 - 375 WP_003413177.1 hypothetical protein -
GS11_RS13180 2813659..2814054 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
GS11_RS13185 2814051..2814296 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
GS11_RS13190 2814707..2815126 - 420 WP_003413190.1 A24 family peptidase -
GS11_RS13195 2815138..2815965 - 828 WP_010950736.1 shikimate dehydrogenase -
GS11_RS13200 2815943..2817196 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
GS11_RS13205 2817189..2817701 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T47388 WP_003413180.1 NZ_CP008744:c2814054-2813659 [Mycobacterium tuberculosis variant bovis BCG]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T47388 NZ_CP008744:c2814054-2813659 [Mycobacterium tuberculosis variant bovis BCG]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT47388 WP_003413183.1 NZ_CP008744:c2814296-2814051 [Mycobacterium tuberculosis variant bovis BCG]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT47388 NZ_CP008744:c2814296-2814051 [Mycobacterium tuberculosis variant bovis BCG]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References