Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2752016..2752668 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag GS11_RS12875 Protein ID WP_003412752.1
Coordinates 2752243..2752668 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag GS11_RS12870 Protein ID WP_003412749.1
Coordinates 2752016..2752237 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS12855 2750256..2750462 - 207 WP_003899347.1 hypothetical protein -
GS11_RS12860 2750598..2751221 + 624 WP_003900858.1 TIGR00725 family protein -
GS11_RS12865 2751211..2751963 + 753 WP_003901416.1 hypothetical protein -
GS11_RS12870 2752016..2752237 + 222 WP_003412749.1 antitoxin Antitoxin
GS11_RS12875 2752243..2752668 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
GS11_RS12880 2752691..2753872 - 1182 WP_010950729.1 2-oxo acid dehydrogenase subunit E2 -
GS11_RS12885 2753869..2754915 - 1047 WP_010950730.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
GS11_RS12890 2754926..2756029 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
GS11_RS12895 2756288..2757109 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
GS11_RS12900 2757106..2757663 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T47384 WP_003412752.1 NZ_CP008744:2752243-2752668 [Mycobacterium tuberculosis variant bovis BCG]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T47384 NZ_CP008744:2752243-2752668 [Mycobacterium tuberculosis variant bovis BCG]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT47384 WP_003412749.1 NZ_CP008744:2752016-2752237 [Mycobacterium tuberculosis variant bovis BCG]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT47384 NZ_CP008744:2752016-2752237 [Mycobacterium tuberculosis variant bovis BCG]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References