Detailed information of TA system TA047379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2216843..2217469
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: GS11_RS10325
• Protein ID: WP_003410075.1
• Coordinates: 2217071..2217469 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GS11_RS10320
• Protein ID: WP_019283586.1
• Coordinates: 2216843..2217070 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS10310	2214882..2215226	-	345	WP_003410065.1	ferredoxin family protein	-
GS11_RS10315	2215415..2216584	-	1170	WP_003899126.1	ATP-binding protein	-
GS11_RS10320	2216843..2217070	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GS11_RS10325	2217071..2217469	+	399	WP_003410075.1	PIN domain nuclease	Toxin
GS11_RS10330	2217652..2218083	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
GS11_RS10335	2218184..2218618	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
GS11_RS21170	2219055..2219234	-	180	Protein_2019	hypothetical protein	-
GS11_RS10340	2219295..2219939	+	645	WP_078396697.1	transposase	-
GS11_RS10345	2219893..2220483	+	591	WP_003899131.1	IS110 family transposase	-
GS11_RS10350	2220611..2221867	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T47379 WP_003410075.1 NZ_CP008744:2217071-2217469 [Mycobacterium tuberculosis variant bovis BCG]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T47379 NZ_CP008744:2217071-2217469 [Mycobacterium tuberculosis variant bovis BCG]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT47379 WP_019283586.1 NZ_CP008744:2216843-2217070 [Mycobacterium tuberculosis variant bovis BCG]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT47379 NZ_CP008744:2216843-2217070 [Mycobacterium tuberculosis variant bovis BCG]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG