Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2193103..2193689 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag GS11_RS10225 Protein ID WP_003410010.1
Coordinates 2193103..2193447 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag GS11_RS10230 Protein ID WP_003410014.1
Coordinates 2193441..2193689 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS10195 2190004..2190309 + 306 Protein_1989 ABC transporter permease -
GS11_RS10200 2190493..2191017 + 525 WP_011799234.1 methyltransferase -
GS11_RS10205 2191537..2192097 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
GS11_RS10210 2192094..2192435 - 342 WP_003410003.1 DUF2384 domain-containing protein -
GS11_RS10215 2192521..2192778 + 258 WP_003410006.1 hypothetical protein -
GS11_RS10220 2192679..2193014 - 336 WP_003410009.1 dehydrogenase -
GS11_RS10225 2193103..2193447 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
GS11_RS10230 2193441..2193689 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
GS11_RS10235 2193789..2196104 - 2316 WP_003899120.1 cation transporter ATPase CptG -
GS11_RS10240 2196101..2196373 - 273 WP_003410017.1 DUF1490 family protein -
GS11_RS10245 2196426..2196782 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
GS11_RS10250 2196939..2197706 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T47378 WP_003410010.1 NZ_CP008744:c2193447-2193103 [Mycobacterium tuberculosis variant bovis BCG]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T47378 NZ_CP008744:c2193447-2193103 [Mycobacterium tuberculosis variant bovis BCG]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT47378 WP_003410014.1 NZ_CP008744:c2193689-2193441 [Mycobacterium tuberculosis variant bovis BCG]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT47378 NZ_CP008744:c2193689-2193441 [Mycobacterium tuberculosis variant bovis BCG]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References