Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1570798..1571453 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag GS11_RS07400 Protein ID WP_003407268.1
Coordinates 1570798..1571199 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag GS11_RS07405 Protein ID WP_003407272.1
Coordinates 1571196..1571453 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS07385 1566642..1568027 - 1386 WP_003407260.1 cytochrome P450 -
GS11_RS07390 1568105..1569139 + 1035 WP_003407264.1 AraC family transcriptional regulator -
GS11_RS07395 1569185..1570543 - 1359 WP_010950541.1 PE family protein -
GS11_RS07400 1570798..1571199 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
GS11_RS07405 1571196..1571453 - 258 WP_003407272.1 ribbon-helix-helix domain-containing protein Antitoxin
GS11_RS07410 1571536..1572495 - 960 WP_011799179.1 alpha/beta hydrolase -
GS11_RS07415 1572520..1573482 - 963 WP_003407279.1 alpha/beta hydrolase -
GS11_RS07420 1573481..1574218 + 738 WP_031647000.1 lysoplasmalogenase -
GS11_RS07425 1574299..1576266 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T47369 WP_003407268.1 NZ_CP008744:c1571199-1570798 [Mycobacterium tuberculosis variant bovis BCG]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T47369 NZ_CP008744:c1571199-1570798 [Mycobacterium tuberculosis variant bovis BCG]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT47369 WP_003407272.1 NZ_CP008744:c1571453-1571196 [Mycobacterium tuberculosis variant bovis BCG]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT47369 NZ_CP008744:c1571453-1571196 [Mycobacterium tuberculosis variant bovis BCG]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References