Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1232421..1233052 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag GS11_RS05855 Protein ID WP_003405820.1
Coordinates 1232421..1232732 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag GS11_RS05860 Protein ID WP_003405836.1
Coordinates 1232732..1233052 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS05835 1227903..1229327 - 1425 WP_003405805.1 class II fumarate hydratase -
GS11_RS05840 1229358..1230446 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
GS11_RS05845 1230502..1231146 + 645 WP_174813088.1 DUF4245 domain-containing protein -
GS11_RS05850 1231153..1232309 - 1157 Protein_1139 AI-2E family transporter -
GS11_RS05855 1232421..1232732 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
GS11_RS05860 1232732..1233052 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
GS11_RS20875 1233062..1234587 + 1526 Protein_1142 carboxylesterase/lipase family protein -
GS11_RS05875 1234605..1235717 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
GS11_RS05880 1235727..1235984 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
GS11_RS05885 1235974..1237221 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
GS11_RS05890 1237218..1237856 - 639 WP_011799158.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T47364 WP_003405820.1 NZ_CP008744:c1232732-1232421 [Mycobacterium tuberculosis variant bovis BCG]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T47364 NZ_CP008744:c1232732-1232421 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT47364 WP_003405836.1 NZ_CP008744:c1233052-1232732 [Mycobacterium tuberculosis variant bovis BCG]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT47364 NZ_CP008744:c1233052-1232732 [Mycobacterium tuberculosis variant bovis BCG]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References