Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 717637..718286 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag GS11_RS03270 Protein ID WP_003403236.1
Coordinates 717891..718286 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag GS11_RS03265 Protein ID WP_003403235.1
Coordinates 717637..717891 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS22285 712762..712845 + 84 Protein_640 galactose-1-phosphate uridylyltransferase -
GS11_RS03240 712864..713946 + 1083 WP_031652152.1 galactose-1-phosphate uridylyltransferase -
GS11_RS03245 713943..715034 + 1092 WP_003403225.1 galactokinase -
GS11_RS03255 715429..716586 + 1158 WP_003903091.1 hypothetical protein -
GS11_RS03260 716597..717544 + 948 WP_003403232.1 DUF732 domain-containing protein -
GS11_RS03265 717637..717891 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
GS11_RS03270 717891..718286 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
GS11_RS03275 718380..719120 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
GS11_RS03280 719252..719512 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GS11_RS03285 719509..719916 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
GS11_RS03290 719988..721139 - 1152 WP_003403248.1 FIST C-terminal domain-containing protein -
GS11_RS03295 721232..722959 - 1728 WP_010950423.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T47354 WP_003403236.1 NZ_CP008744:717891-718286 [Mycobacterium tuberculosis variant bovis BCG]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T47354 NZ_CP008744:717891-718286 [Mycobacterium tuberculosis variant bovis BCG]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT47354 WP_003403235.1 NZ_CP008744:717637-717891 [Mycobacterium tuberculosis variant bovis BCG]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT47354 NZ_CP008744:717637-717891 [Mycobacterium tuberculosis variant bovis BCG]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References